Evidence map›Paper›PMID 26689537›Full record

ArticleInternational journal of analytical chemistry2015

Highly Sensitive Immunochromatographic Identification of Tetracycline Antibiotics in Milk.

N A Taranova, A S Kruhlik, E A Zvereva, V V Shmanai, I I Vashkevich, D A Semyonov, S A Eremin, A V Zherdev, B B Dzantiev

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In one paragraph

Article in International journal of analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

N A TaranovaA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia.
A S KruhlikInstitute of Physical Organic Chemistry, Surganov Street 13, 220072 Minsk, Belarus.
E A ZverevaA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia.
V V ShmanaiInstitute of Physical Organic Chemistry, Surganov Street 13, 220072 Minsk, Belarus.
I I VashkevichInstitute of Bioorganic Chemistry, Acad. Kuprevich Street 5/2, 220141 Minsk, Belarus.
D A SemyonovInstitute of Bioorganic Chemistry, Acad. Kuprevich Street 5/2, 220141 Minsk, Belarus.
S A EreminA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia ; Chemical Department, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russia.
A V ZherdevA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia.
B B DzantievA.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia.
A N Bach Institute of Biochemistry · RUInstitute of Bioorganic Chemistry · BYInstitute of Physical and Organic Chemistry · BY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A rapid immunochromatographic assay was developed for the control of tetracycline (TC). The assay is based on the competition between immobilized TC-protein conjugate and TC in a tested sample for binding with polyclonal anti-TC antibodies conjugated to colloidal gold during the flow of the sample along a membrane strip with immobilized reactants. Conjugation of colloidal gold and the total immunoglobulin (IgG) fraction of polyclonal antibodies was used to increase the assay sensitivity to ensure low content of specific antibodies in the conjugate. This allowed effective inhibition of free TC and conjugate binding in the strip test zone. Photometric marker registration allows control of the reduction of binding, thereby enhancing detection sensitivity. The proposed assay allows TC to be detected at concentrations up to 20 ng/mL, exceeding the limit of detection of the known analogues, in a wide working range (more than two orders) of 60 pg/mL to 10 ng/mL, ensured through the use of polyclonal antibodies. The assay time is 10 min. The efficiency of the designed assay is shown to identify TC in milk; the degree of recovery of TC ranges from 90 to 112%. The precision of the concentrations measurements was no more than 10%.

Identifiers

PMID26689537
PMCPMC4673384
OpenAlexW2174625737

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.