ArticlePloS one2015
In Vitro Evaluation of Spider Silk Meshes as a Potential Biomaterial for Bladder Reconstruction.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Population Kinetics and Protein Profiles of Co-Cultured Adult and Fetus Rabbit Bladder Smooth Muscle Cells.Urology research & practice · 2025Article
- Engineering with Biomedical Sciences Changing the Horizon of Healthcare-A Review.Bioengineered · 2024Review
- Bacteria inhabiting spider webs enhance host silk extensibility.Scientific reports · 2024Article
- Review of Spider Silk Applications in Biomedical and Tissue Engineering.Biomimetics (Basel, Switzerland) · 2024Review
- Article
- Review
- Non-Muscular Invasive Bladder Cancer: Re-envisioning Therapeutic Journey from Traditional to Regenerative Interventions.Aging and disease · 2021Review
- Defining the regenerative effects of native spider silk fibers on primary Schwann cells, sensory neurons, and nerve-associated fibroblasts.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Spider Silk for Tissue Engineering Applications.Molecules (Basel, Switzerland) · 2020Review
- Protein Nanotubes: From Bionanotech towards Medical Applications.Biomedicines · 2019Review
- Piezoresponse, Mechanical, and Electrical Characteristics of Synthetic Spider Silk Nanofibers.Nanomaterials (Basel, Switzerland) · 2018Article
- miR-33a hinders the differentiation of adipose mesenchymal stem cells towards urothelial cells in an inductive condition by targeting β‑catenin and TGFR.Molecular medicine reports · 2018Article
- Influence of direct or indirect contact for the cytotoxicity and blood compatibility of spider silk.Journal of materials science. Materials in medicine · 2017Article
- Structural and Mechanical Properties of Amyloid Beta Fibrils: A Combined Experimental and Theoretical Approach.The journal of physical chemistry letters · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reconstruction of the bladder by means of both natural and synthetic materials remains a challenge due to severe adverse effects such as mechanical failure. Here we investigate the application of spider major ampullate gland-derived dragline silk from the Nephila edulis spider, a natural biomaterial with outstanding mechanical properties and a slow degradation rate, as a potential scaffold for bladder reconstruction by studying the cellular response of primary bladder cells to this biomaterial. We demonstrate that spider silk without any additional biological coating supports adhesion and growth of primary human urothelial cells (HUCs), which are multipotent bladder cells able to differentiate into the various epithelial layers of the bladder. HUCs cultured on spider silk did not show significant changes in the expression of various epithelial-to-mesenchymal transition and fibrosis associated genes, and demonstrated only slight reduction in the expression of adhesion and cellular differentiation genes. Furthermore, flow cytometric analysis showed that most of the silk-exposed HUCs maintain an undifferentiated immunophenotype. These results demonstrate that spider silk from the Nephila edulis spider supports adhesion, survival and growth of HUCs without significantly altering their cellular properties making this type of material a suitable candidate for being tested in pre-clinical models for bladder reconstruction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.