ArticleToxicology in vitro : an international journal published in association with BIBRA2016
Phthalate metabolism and kinetics in an in vitro model of testis development.
Article in Toxicology in vitro : an international journal published in association with BIBRA, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- The effects of gene × environment interactions on silver nanoparticle toxicity in the respiratory system: An adverse outcome pathway.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2021Review
- Phthalates in Albumin from Human Serum: Implications for Assisted Reproductive Technology.F&S reviews · 2021Article
- The Variations of Metabolic Detoxification Enzymes Lead to Recurrent Miscarriage and Their Diagnosis Strategy.Advances in experimental medicine and biology · 2021Article
- Programming microphysiological systems for children's health protection.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Inhibitory effects of fifteen phthalate esters in human cDNA-expressed UDP-glucuronosyltransferase supersomes.Chemosphere · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
We have developed an in vitro model of testis development (3D-TCS) using rat testicular cells overlaid with extracellular matrix. One barrier preventing utilization of in vitro models in toxicity testing is the absence of metabolic capability. Another challenge is lack of kinetic data for compounds in vitro. We characterized metabolic capabilities and investigated the kinetics of phthalate male reproductive toxicants in the 3D-TCS. Cells were treated with three phthalate diesters for 2, 8 and 24 h. Parent compounds and metabolites were measured in cell culture media and cell lysate via mass spectrometry. Levels of monoester metabolites were used as an indication of metabolism of phthalates via lipase activity. Metabolites were detected in all treated cell media and cell lysate samples, with levels ranging from <0.5-14.7% of initial mass of parent compound. Phthalates partitioned between media and lysate in a manner consistent with each compound's degree of lipophilicity. UDGPT activity was detected in DBP and DEP treated samples. 3D-TCS microarray data indicated gene expression for lipases and CYPP450s. Results indicate that the 3D-TCS is a metabolically active co-culture and that physiochemical properties can provide information about the kinetics of compounds in the 3D-TCS, improving our ability to interpret results from the model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.