ArticlePlant methods2015
Fast virtual histology using X-ray in-line phase tomography: application to the 3D anatomy of maize developing seeds.
Article in Plant methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Integrated physiological and transcriptomic measurements reveal changes in the accumulation and partitioning of storage reserves after prevention of pollination in maize.BMC plant biology · 2026Article
- Laboratory-based cellular-level correlative visible-light and X-ray microscopy for 3D evaluation of mouse kidney biopsy.Scientific reports · 2026Article
- A Multidimensional Approach to Cereal Caryopsis Development: Insights into Adlay (Plants (Basel, Switzerland) · 2026Article
- Evaluation of 3D seed structure and cellular traits in-situ using X-ray microscopy.Scientific reports · 2025Article
- Leveraging sensor technologies for seed phenotyping by genebanks.Frontiers in plant science · 2025Article
- Precise 3D geometric phenotyping and phenotype interaction network construction of maize kernels.Frontiers in plant science · 2025Article
- Metabolic imaging in living plants: A promising field for chemical exchange saturation transfer (CEST) MRI.Science advances · 2024Article
- Non-Destructive Assessment of Microstructural Changes in Kabuli Chickpeas during Storage.Foods (Basel, Switzerland) · 2024Article
- Visualization and Quantitative Evaluation of Functional Structures of Soybean Root Nodules via Synchrotron X-ray Imaging.Plant phenomics (Washington, D.C.) · 2024Article
- Applications of synchrotron light in seed research: an array of x-ray and infrared imaging methodologies.Frontiers in plant science · 2024Article
- New Growth-Related Features of Wheat Grain Pericarp Revealed by Synchrotron-Based X-ray Micro-Tomography and 3D Reconstruction.Plants (Basel, Switzerland) · 2023Article
- Size measurement and filled/unfilled detection of rice grains using backlight image processing.Frontiers in plant science · 2023Article
- Automated extraction of pod phenotype data from micro-computed tomography.Frontiers in plant science · 2023Article
- Compact laboratory-based X-ray microscope enabling nondestructive 3D structure acquisition of mouse nephron with high speed and better user accessibility.Microscopy (Oxford, England) · 2022Article
- Synchrotron tomography applications in agriculture and food sciences research: a review.Plant methods · 2022Review
- Nondestructive cellular-level 3D observation of mouse kidney using laboratory-based X-ray microscopy with paraffin-mediated contrast enhancement.Scientific reports · 2022Article
- Article
- Grain filling leads to backflow of surplus water from the maize grain to the cob and plantFrontiers in plant science · 2022Article
- Lipid anchoring and electrostatic interactions target NOT-LIKE-DAD to pollen endo-plasma membrane.The Journal of cell biology · 2021Article
- Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum.The Plant cell · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various success, depending on the size and physical properties of the biological material.
resultsX-ray in-line phase tomography was applied for the imaging of internal structures of maize seeds at early stages of development, when the cells are metabolically fully active and water is the main cell content. This 3D imaging technique with histology-like spatial resolution is demonstrated to reveal the anatomy of seed compartments with unequalled contrast by comparison with X-ray absorption tomography. An associated image processing pipeline allowed to quantitatively segment in 3D the four compartments of the seed (embryo, endosperm, nucellus and pericarp) from 7 to 21 days after pollination.
conclusionThis work constitutes an innovative quantitative use of X-ray in-line phase tomography as a non-destructive fast method to perform virtual histology and extends the developmental stages accessible by this technique which had previously been applied in seed biology to more mature samples.
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