ArticleMitochondrion2016
Fully automated software for quantitative measurements of mitochondrial morphology.
Article in Mitochondrion, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Impairment of Neuronal Mitochondrial Quality Control in Prion-Induced Neurodegeneration.Cells · 2022Article
- Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks.ACS biomaterials science & engineering · 2020Article
- p32/C1QBP regulates OMA1-dependent proteolytic processing of OPA1 to maintain mitochondrial connectivity related to mitochondrial dysfunction and apoptosis.Scientific reports · 2020Article
- Mitochondrial Movement and Number Deficits in Embryonic Cortical Neurons from 3xTg-AD Mice.Journal of Alzheimer's disease : JAD · 2019Article
- Acute Nitric Oxide Synthase Inhibition Accelerates Transendothelial Insulin Efflux In Vivo.Diabetes · 2018Article
- Connecting mitochondrial dynamics and life-or-death events via Bcl-2 family proteins.Neurochemistry international · 2017Review
- A novel algorithm identifies stress-induced alterations in mitochondrial connectivity and inner membrane structure from confocal images.PLoS computational biology · 2017Article
- Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology.Journal of neurovirology · 2016Article
- Raw and processed microscope images of fixed cells at baseline and following various experimental perturbations.Data in brief · 2016Article
- Fully automated software for quantitative measurements of mitochondrial morphology.Mitochondrion · 2016Article
- Mitochondria in smooth muscle cells of viscera.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshiArticle
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Authors and funding
5 authors.
Funding
Abstract
Mitochondria undergo dynamic changes in morphology in order to adapt to changes in nutrient and oxygen availability, communicate with the nucleus, and modulate intracellular calcium dynamics. Many recent papers have been published assessing mitochondrial morphology endpoints. Although these studies have yielded valuable insights, contemporary assessment of mitochondrial morphology is typically subjective and qualitative, precluding direct comparison of outcomes between different studies and likely missing many subtle effects. In this paper, we describe a novel software technique for measuring the average length, average width, spatial density, and intracellular localization of mitochondria from a fluorescent microscope image. This method was applied to distinguish baseline characteristics of Human Umbilical Vein Endothelial Cells (HUVECs), primary Goto-Kakizaki rat aortic smooth muscle cells (GK SMCs), primary Wistar rat aortic smooth muscle cells (Wistar SMCs), and SH-SY5Ys (human neuroblastoma cell line). Consistent with direct observation, our algorithms found SH-SY5Ys to have the greatest mitochondrial density, while HUVECs were found to have the longest mitochondria. Mitochondrial morphology responses to temperature, nutrient, and oxidative stressors were characterized to test algorithm performance. Large morphology changes recorded by the software agreed with direct observation, and subtle but consistent morphology changes were found that would not otherwise have been detected. Endpoints were consistent between experimental repetitions (R=0.93 for length, R=0.93 for width, R=0.89 for spatial density, and R=0.74 for localization), and maintained reasonable agreement even when compared to images taken with compromised microscope resolution or in an alternate imaging plane. These results indicate that the automated software described herein allows quantitative and objective characterization of mitochondrial morphology from fluorescent microscope images.
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