Evidence map›Paper›PMID 26687918›Full record

ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2016

The interplay between autophagy and apoptosis induced by tanshinone IIA in prostate cancer cells.

Chunlong Li, Xiancheng Han, Hong Zhang, Jinsheng Wu, Bao Li

Abstract read
PubMed Publisher
In one paragraph

Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
6.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. A review of tanshinone compounds in prostate cancer treatment.Translational andrology and urology · 2024
    Review
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  12. An overview of the anti-cancer actions of Tanshinones, derived fromExploration of targeted anti-tumor therapy · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Chunlong LiUrological Department, Affiliate Hospital of Weifang Medical University, No.2428 Yuhe Road, Weifang, Shandong, China. li_cl88@126.com.
Xiancheng HanUrological Department, Affiliate Hospital of Weifang Medical University, No.2428 Yuhe Road, Weifang, Shandong, China.
Hong ZhangUrological Department, Affiliate Hospital of Weifang Medical University, No.2428 Yuhe Road, Weifang, Shandong, China.
Jinsheng WuUrological Department, Affiliate Hospital of Weifang Medical University, No.2428 Yuhe Road, Weifang, Shandong, China.
Bao LiUrological Department, Affiliate Hospital of Weifang Medical University, No.2428 Yuhe Road, Weifang, Shandong, China.
Weifang Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tanshinone IIA (T2A), a derivative of phenanthrenequinone and also the major active ingredient of Danshen, has been paid extensive attention as a promising cancer therapy for its potential anti-cancer activities. In this study, the apoptosis and autophagy of human prostate cancer PC-3 cells were observed after 5 μM T2A treatment, as well as their relevance. Mitochondrial-dependent apoptosis was firstly detected through morphological observation and biochemical analysis. Meanwhile, 5 μM T2A successfully triggered the autophagy of PC-3 cells, indicated by increased expression of Beclin1, and LC3 II. Validation experiments were conducted to further consolidate T2A's contribution to autophagy: Pretreatment with autophagy inhibitor 3-methyladenine (3-MA) provided protection against autophagy and enhanced T2A-induced apoptosis. Besides, the apoptosis suppressor z-VAD-fmk failed to facilitate the formation of autophagic vacuoles, which also proved the T2A-induced autophagy independent of apoptosis. Moreover, the reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine (NAC) efficiently inhibited the expression of Beclin1, LC3-II, and cleaved caspase-3, which indicated apoptosis and autophagy with dependence on intracellular ROS production. Taken together, these results demonstrated that autophagy is the cytoprotective mechanism in this experimental system, and the ROS resulted from T2A treatment played a critical role in apoptosis and autophagy initiation.

Indexed as

AbietanesAcetylcysteineAdenocarcinomaAmino Acid Chloromethyl KetonesAntineoplastic Agents, PhytogenicApoptosisAutophagyBeclin-1Caspase 3Cell Line, TumorDrug Screening Assays, AntitumorHumansMaleMicrotubule-Associated ProteinsNeoplasm ProteinsProstatic NeoplasmsAbietanesAcetylcysteineAmino Acid Chloromethyl KetonesAntineoplastic Agents, PhytogenicBeclin-1BECN1 protein, humanbenzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketoneCASP3 protein, humanCaspase 3MAP1LC3A protein, humanMicrotubule-Associated ProteinsNeoplasm ProteinsReactive Oxygen SpeciestanshinoneApoptosisAutophagyProstate cancerReactive oxygen speciesTanshinone IIA

Identifiers

PMID26687918
OpenAlexW2300907231

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.