Evidence map›Paper›PMID 26687789›Full record

ArticleImmunogenetics2016

Refinement of the canine CD1 locus topology and investigation of antibody binding to recombinant canine CD1 isoforms.

Mette Schjaerff, Stefan M Keller, Joseph Fass, Lutz Froenicke, Robert A Grahn, Leslie Lyons, Verena K Affolter, Annemarie T Kristensen, Peter F Moore

Abstract read
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In one paragraph

Article in Immunogenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Role of NK-Like CD8Infection and immunity · 2022
    Article
  2. Mammalian CD1 and MR1 genes.Immunogenetics · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Mette SchjaerffDepartment of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg C, Denmark.
Stefan M KellerDepartment of Veterinary Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Joseph FassUC Davis Genome Center - Bioinformatics Core, University of California, 451 Health Science Drive, Davis, CA, 95616, USA.
Lutz FroenickeUC Davis Genome Center - DNA Technologies and Expression Analysis Cores, University of California, 451 Health Science Drive, Davis, CA, 95616, USA.
Robert A GrahnVeterinary Genetics Laboratory, University of California, 980 Old Davis Road, Davis, CA, 95616, USA.
Leslie LyonsLyons Feline and Comparative Genetics, Department of Veterinary Medicine and Surgery, University of Missouri, 1600 E Rollins Street, Colombia, MO, 65211, USA.
Verena K AffolterDepartment of Veterinary Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Annemarie T KristensenDepartment of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg C, Denmark.
Peter F MooreDepartment of Veterinary Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA, 95616, USA. pfmoore@ucdavis.edu.
University of California, Davis · USUniversity of Copenhagen · DKUniversity of Missouri · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CD1 molecules are antigen-presenting glycoproteins primarily found on dendritic cells (DCs) responsible for lipid antigen presentation to CD1-restricted T cells. Despite their pivotal role in immunity, little is known about CD1 protein expression in dogs, notably due to lack of isoform-specific antibodies. The canine (Canis familiaris) CD1 locus was previously found to contain three functional CD1A genes: canCD1A2, canCD1A6, and canCD1A8, where two variants of canCD1A8, canCD1A8.1 and canCD1A8.2, were assumed to be allelic variants. However, we hypothesized that these rather represented two separate genes. Sequencing of three overlapping bacterial artificial chromosomes (BACs) spanning the entire canine CD1 locus revealed canCD1A8.2 and canCD1A8.1 to be located in tandem between canCD1A7 and canCD1C, and canCD1A8.1 was consequently renamed canCD1A9. Green fluorescent protein (GFP)-fused canine CD1 transcripts were recombinantly expressed in 293T cells. All proteins showed a highly positive GFP expression except for canine CD1d and a splice variant of canine CD1a8 lacking exon 3. Probing with a panel of anti-CD1 monoclonal antibodies (mAbs) showed that Ca13.9H11 and Ca9.AG5 only recognized canine CD1a8 and CD1a9 isoforms, and Fe1.5F4 mAb solely recognized canine CD1a6. Anti-CD1b mAbs recognized the canine CD1b protein, but also bound CD1a2, CD1a8, and CD1a9. Interestingly, Ca9.AG5 showed allele specificity based on a single nucleotide polymorphism (SNP) located at position 321. Our findings have refined the structure of the canine CD1 locus and available antibody specificity against canine CD1 proteins. These are important fundamentals for future investigation of the role of canine CD1 in lipid immunity.

Indexed as

Genetic LociRecombinant Fusion ProteinsAllelesAmino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityAntigens, CD1Base SequenceComputational BiologyDogsMolecular Sequence DataPolymorphism, Single NucleotideProtein BindingProtein IsoformsSequence AlignmentAntibodies, MonoclonalAntigens, CD1Protein IsoformsRecombinant Fusion ProteinsAntibody epitope identificationAntibody recognition of canine CD1Canine CD1 genesCanine CD1 proteinsCanine dendritic cellsSingle nucleotide polymorphisms (SNPs)

Identifiers

PMID26687789
OpenAlexW2285787522

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.