ArticleMicrobiologyOpen2016
Transcriptomic profiling of Methylococcus capsulatus (Bath) during growth with two different methane monooxygenases.
Article in MicrobiologyOpen, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- A Study of the Community Relationships Between Methanotrophs and Their Satellites Using Constraint-Based Modeling Approach.International journal of molecular sciences · 2024Article
- Overexpression of native carbonic anhydrases increases carbon conversion efficiency in the methanotrophic biocatalystmSphere · 2024Article
- MmoD regulates soluble methane monooxygenase and methanobactin production inApplied and environmental microbiology · 2023Article
- Transcriptional and metabolomic responses ofFrontiers in microbiology · 2023Article
- Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds.Advances in biochemical engineering/biotechnology · 2022Article
- Expanding Characterized Diversity and the Pool of Complete Genome Sequences ofFrontiers in microbiology · 2021Article
- Thriving in Wetlands: Ecophysiology of the Spiral-Shaped MethanotrophMicroorganisms · 2019Article
- MbnH is a diheme MauG-like protein associated with microbial copper homeostasis.The Journal of biological chemistry · 2019Article
- PCuThe Journal of biological chemistry · 2019Article
- Article
- A Mutagenic Screen Identifies a TonB-Dependent Receptor Required for the Lanthanide Metal Switch in the Type I Methanotroph "Methylotuvimicrobium buryatense" 5GB1C.Journal of bacteriology · 2019Article
- Development of a CRISPR/Cas9 System for Methylococcus capsulatusApplied and environmental microbiology · 2019Article
- Genome-scale metabolic reconstruction and metabolic versatility of an obligate methanotrophPeerJ · 2019Article
- Efficient Counterselection for Methylococcus capsulatus (Bath) by Using a MutatedApplied and environmental microbiology · 2018Article
- Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria.Nature communications · 2018Article
- Chalkophores.Annual review of biochemistry · 2018Review
- Methanobactins: Maintaining copper homeostasis in methanotrophs and beyond.The Journal of biological chemistry · 2018Review
- Metals and Methanotrophy.Applied and environmental microbiology · 2018Review
- Extracellular Electron Transfer via Outer Membrane Cytochromes in a Methanotrophic BacteriumFrontiers in microbiology · 2018Article
- A Genome-Scale Metabolic Model forFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). The expression of these two MMO's is tightly controlled by the availability of copper in the growth medium, but the underlying mechanisms and the number of genes involved in this switch in methane oxidation is not yet fully elucidated. Microarray analyses were used to assess the transcriptome in cells producing either pMMO or sMMO. A total of 137 genes were differentially expressed, with 87 genes showing a significant up-regulation during sMMO production. The majority of the differentially expressed genes could be assigned to functional roles in the energy metabolism and transport. Furthermore, three copper responding gene clusters were discovered, including an extended cluster that also harbors the genes for sMMO. Our data also indicates that major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells, and that quinone are predominantly used as the electron donors for methane oxidation by pMMO. Intriguingly, a large proportion of the differentially expressed genes between pMMO- and sMMO-producing cells encode c-type cytochromes. By combining microarray- and mass spectrometry data, a total of 35 c-type cytochromes are apparently expressed in M. capsulatus when grown in nitrate mineral salt medium with methane as sole energy and carbon source, and the expression of 21 of these respond to the availability of copper. Interestingly, several of these c-type cytochromes are recovered from the cell surface, suggesting that extracellular electron transfers may occur in M. capsulatus.
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Registered trials
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