Evidence map›Paper›PMID 26687464›Full record

ArticleCurrent microbiology2016

SDS-PAGE and Gel IEF: Tool for Differentiation of Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus aureus.

Marie Tesařová, Marie Horká, Dana Moravcová, Lenka Svojanovská, Katarina Mlynarikova, Filip Růžička

Abstract readEvaluation Study
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In one paragraph

Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marie TesařováInstitute of Analytical Chemistry of the CAS, v. v. i., Veveří 97, 602 00, Brno, Czech Republic. tesarova@iach.cz.
Marie HorkáInstitute of Analytical Chemistry of the CAS, v. v. i., Veveří 97, 602 00, Brno, Czech Republic.
Dana MoravcováInstitute of Analytical Chemistry of the CAS, v. v. i., Veveří 97, 602 00, Brno, Czech Republic.
Lenka SvojanovskáInstitute of Analytical Chemistry of the CAS, v. v. i., Veveří 97, 602 00, Brno, Czech Republic.
Katarina MlynarikovaThe Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 53/5, 625 00, Brno, Czech Republic.
Filip RůžičkaThe Department of Microbiology, Faculty of Medicine, Masaryk University, Kamenice 53/5, 625 00, Brno, Czech Republic.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The methicillin-resistant Staphylococcus aureus causes difficult-to-treat healthcare-associated infections in humans. For fast and effective selection of an appropriate antibiotic therapy, it is essential to have rapid and reliable methods for differentiation of methicillin-resistant S. aureus from less dangerous methicillin-sensitive S. aureus. There have been many methods for the identification of methicillin-resistant S. aureus described but none has been accepted as an international standard. The most commonly used techniques such as phenotyping and genotyping have a few disadvantages, for instance, these techniques are not reproducible and stable. In addition, they are time-consuming, expensive, and they are not capable to distinguish all S. aureus strains. In this study, the methicillin-resistant and methicillin-sensitive S. aureus isolates obtained from patients were extracted in hot water. The released proteins were characterised by sodium dodecyl sulphate polyacrylamide gel electrophoresis and gel isoelectric focusing. These two methods were able to differentiate among tested bacterial strains. The proposed methods are time saving, they are applicable in standard biochemical laboratories, and they do not require any expensive equipment.

Indexed as

Methicillin ResistanceBacteriological TechniquesElectrophoresis, Polyacrylamide GelHumansIsoelectric FocusingStaphylococcus aureusTime Factors

Identifiers

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.