ArticleScientific reports2015
Antibiotic regimen based on population analysis of residing persister cells eradicates Staphylococcus epidermidis biofilms.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Assembling the puzzle of antimicrobial resistance in staphylococcal biofilms.Emerging microbes & infections · 2026Article
- Using biofilm-targeting local antibiotics to treat recalcitrant medical device-associated infections: driveline infection as a model.The Journal of antimicrobial chemotherapy · 2026Article
- Antimicrobial Effect of Spices and Their Phytochemicals: A Novel Approach to Overcoming Antibiotic Resistance.MedComm · 2026Review
- Marine Antimicrobial Peptides: Emerging Strategies Against Multidrug-Resistant and Biofilm-Forming Bacteria.Antibiotics (Basel, Switzerland) · 2025Review
- Nanowire arrays with programmable geometries as a highly effective anti-biofilm surface.Biofilm · 2025Article
- Repurposing antimicrobials with ultrasound-triggered nanoscale systems for targeted biofilm drug delivery.npj antimicrobials and resistance · 2025Article
- Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms.AMB Express · 2024Article
- Molecular mechanism and application of emerging technologies in study of bacterial persisters.BMC microbiology · 2024Review
- Antibiofilm activity of marine microbial natural products: potential peptide- and polyketide-derived molecules from marine microbes toward targeting biofilm-forming pathogens.Journal of natural medicines · 2024Review
- Overcoming Antibiotic Resistance with Novel Paradigms of Antibiotic Selection.Microorganisms · 2022Review
- Ventricular Assist Device-Specific Infections.Journal of clinical medicine · 2021Review
- Antimicrobial Activities of Alginate and Chitosan Oligosaccharides AgainstFrontiers in microbiology · 2021Article
- Combination Therapy to Treat Fungal Biofilm-Based Infections.International journal of molecular sciences · 2020Review
- The Protective Effect ofViruses · 2020Article
- Biofilm Formation ofFrontiers in microbiology · 2020Article
- RAFT-Derived Polymethacrylates as a Superior Treatment for Recurrent Vulvovaginal Candidiasis by Targeting Biotic Biofilms and Persister Cells.Frontiers in microbiology · 2019Article
- Multidrug efflux pumps: structure, function and regulation.Nature reviews. Microbiology · 2018Review
- How do environment-dependent switching rates between susceptible and persister cells affect the dynamics of biofilms faced with antibiotics?NPJ biofilms and microbiomes · 2018Article
- Efflux drug transporters at the forefront of antimicrobial resistance.European biophysics journal : EBJ · 2017Review
- Efficient Killing of Planktonic and Biofilm-Embedded Coagulase-Negative Staphylococci by Bactericidal Protein P128.Antimicrobial agents and chemotherapy · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm formation is a major pathogenicity strategy of Staphylococcus epidermidis causing various medical-device infections. Persister cells have been implicated in treatment failure of such infections. We sought to profile bacterial subpopulations residing in S. epidermidis biofilms, and to establish persister-targeting treatment strategies to eradicate biofilms. Population analysis was performed by challenging single biofilm cells with antibiotics at increasing concentrations ranging from planktonic minimum bactericidal concentrations (MBCs) to biofilm MBCs (MBCbiofilm). Two populations of "persister cells" were observed: bacteria that survived antibiotics at MBCbiofilm for 24/48 hours were referred to as dormant cells; those selected with antibiotics at 8 X MICs for 3 hours (excluding dormant cells) were defined as tolerant-but-killable (TBK) cells. Antibiotic regimens targeting dormant cells were tested in vitro for their efficacies in eradicating persister cells and intact biofilms. This study confirmed that there are at least three subpopulations within a S. epidermidis biofilm: normal cells, dormant cells, and TBK cells. Biofilms comprise more TBK cells and dormant cells than their log-planktonic counterparts. Using antibiotic regimens targeting dormant cells, i.e. effective antibiotics at MBCbiofilm for an extended period, might eradicate S. epidermidis biofilms. Potential uses for this strategy are in antibiotic lock techniques and inhaled aerosolized antibiotics.
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