Evidence map›Paper›PMID 26686232›Full record

ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2016

Effect of different culture media and deswelling agents on survival of human corneal endothelial and epithelial cells in vitro.

Monika Valtink, Patricia Donath, Katrin Engelmann, Lilla Knels

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Monika ValtinkInstitute of Anatomy, Faculty of Medicine Carl Gustav Carus of the TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany. monika.valtink@tu-dresden.de.ORCID http://orcid.org/0000-0003-3205-1876
Patricia DonathInstitute of Anatomy, Faculty of Medicine Carl Gustav Carus of the TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany.
Katrin EngelmannDepartment of Ophthalmology, Klinikum Chemnitz gGmbH, Flemmingstr. 2, 09116, Chemnitz, Germany.
Lilla KnelsInstitute of Anatomy, Faculty of Medicine Carl Gustav Carus of the TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany.
Technische Universität Dresden · DEKlinikum Chemnitz · DEUniversity Hospital Carl Gustav Carus · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo examine the effects of media and deswelling agents on human corneal endothelial and epithelial cell viability using a previously developed screening system.

methodsThe human corneal endothelial cell line HCEC-12 and the human corneal epithelial cell line HCE-T were cultured in four different corneal organ culture media (serum-supplemented: MEM +2 % FCS, CorneaMax®/CorneaJet®, serum-free: Human Endothelial-SFM, Stemalpha-2 and -3) with and without 6 % dextran T500 or 7 % HES 130/0.4. Standard growth media F99HCEC and DMEM/F12HCE-T served as controls. In additional controls, the stress inducers staurosporine or hydrogen peroxide were added. After 5 days in the test media, cell viability was assessed by flow cytometrically quantifying apoptotic and necrotic cells (sub-G1 DNA content, vital staining with YO-PRO-1® and propidium iodide) and intracellular reactive oxygen species (ROS).

resultsThe MEM-based media were unable to support HCEC-12 and HCE-T survival under stress conditions, resulting in significantly increased numbers of apoptotic and necrotic cells. HCEC-12 survival was markedly improved in SFM-based media even under staurosporine or hydrogen peroxide. Likewise, HCE-T survival was improved in SFM with or without dextran. The media CorneaMax®, CorneaJet®, and CorneaMax® with HES supported HCEC-12 survival better than MEM-based media, but less well than SFM-based media. HCE-T viability was also supported by CorneaJet®, but not by CorneaMax® with or without HES. Stemalpha-based media were not suitable for maintaining viability of HCEC-12 or HCE-T in the applied cell culture system.

conclusionsThe use of serum-supplemented MEM-based media for corneal organ culture should be discontinued in favour of serum-free media like SFM.

Indexed as

ApoptosisCell LineCell SurvivalCulture MediaCulture Media, Serum-FreeDextransEndothelium, CornealEnzyme InhibitorsEpithelium, CornealFlow CytometryHumansHydrogen PeroxideHydroxyethyl Starch DerivativesMicroscopy, Phase-ContrastNecrosisOrgan Culture TechniquesCulture MediaCulture Media, Serum-FreeDextransEnzyme InhibitorsHydrogen PeroxideHydroxyethyl Starch DerivativesOxidantsPlasma SubstitutesReactive Oxygen SpeciesStaurosporineCell survivalCorneal organ cultivationDeswelling substanceHuman corneal endotheliumHuman corneal epitheliumSerum-free medium

Identifiers

PMID26686232
OpenAlexW2209268251

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.