ArticleDigestive diseases and sciences2016
Tight Junction Ultrastructure Alterations in a Mouse Model of Enteral Nutrient Deprivation.
Article in Digestive diseases and sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Adoption of an Early Enteral Nutrition Feeding Protocol in Patients Receiving an Allogeneic Stem Cell Transplant.Nutrients · 2026Article
- Mouse models for enteral and parenteral nutrition after surgery.Frontiers in veterinary science · 2025Article
- Fiber in the ICU: Should it Be a Regular Part of Feeding?Current gastroenterology reports · 2021Review
- The Structure and Function of the Human Small Intestinal Microbiota: Current Understanding and Future Directions.Cellular and molecular gastroenterology and hepatology · 2020Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundTotal parenteral nutrition (TPN), a necessary treatment for patients who cannot receive enteral nutrition, is associated with infectious complications due in part to a loss of intestinal epithelial barrier function (EBF). Using a mouse model of TPN, with enteral nutrient deprivation, we previously demonstrated an increase in mucosal interferon-γ and tumor necrosis factor-α; these cytokine changes are a major mediator driving a reduction in epithelial tight junction (TJ) protein expression. However, the exact ultrastructural changes to the intestinal epithelial barrier have not been previously described.
aimWe hypothesized that TPN dependence results in ultrastructural changes in the intestinal epithelial TJ meshwork.
methodsC57BL/6 mice underwent internal jugular venous cannulation and were given enteral nutrition or TPN with enteral nutrient deprivation for 7 days. Freeze-fracture electron microscopy was performed on ileal tissue to characterize changes in TJ ultrastructure. EBF was measured using transepithelial resistance and tracer permeability, while TJ expression was measured via Western immunoblotting and immunofluorescence staining.
resultsWhile strand density, linearity, and appearance were unchanged, TPN dependence led to a mean reduction in one horizontal strand out of the TJ compact meshwork to a more basal region, resulting in a reduction in meshwork depth. These findings were correlated with the loss of TJ localization of claudin-4 and tricellulin, reduced expression of claudin-5 and claudin-8, and reduced ex vivo EBF.
conclusionTight junction ultrastructural changes may contribute to reduced EBF in the setting of TPN dependence.
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