ArticleJournal of experimental botany2016
Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Iron need attention: a review on the role of iron in plants.Frontiers in plant science · 2026Review
- Innovative metabolic reprogramming in rice: unlocking drought resilience through microbial consortia interaction and sustainable agriculture.3 Biotech · 2025Review
- Altitudinal Variation on Metabolites, Elements, and Antioxidant Activities of Medicinal PlantMetabolites · 2023Article
- Deciphering the functional roles of transporter proteins in subcellular metal transportation of plants.Planta · 2023Review
- Identification and Functional Analysis of Two Mitoferrins, CsMIT1 and CsMIT2, Participating in Iron Homeostasis in Cucumber.International journal of molecular sciences · 2023Article
- Growth Developmental Defects of Mitochondrial Iron Transporter 1 and 2 Mutants in Arabidopsis in Iron Sufficient Conditions.Plants (Basel, Switzerland) · 2023Article
- Insights into the regulation of energy metabolism during the seed-to-seedling transition in marine angiospermFrontiers in plant science · 2023Article
- Review
- Mitochondrial iron transporter (MIT) gene in potato (Solanum tuberosum): comparative bioinformatics, physiological and expression analyses in response to drought and salinity.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Article
- Iron in leaves: chemical forms, signalling, and in-cell distribution.Journal of experimental botany · 2022Review
- Soil chemistry determines whether defensive plant secondary metabolites promote or suppress herbivore growth.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Ferroportin 3 is a dual-targeted mitochondrial/chloroplast iron exporter necessary for iron homeostasis in Arabidopsis.The Plant journal : for cell and molecular biology · 2021Article
- Altered levels of mitochondrial NFS1 affect cellular Fe and S contents in plants.Plant cell reports · 2019Article
- Tolerance of Iron-Deficient and -Toxic Soil Conditions in Rice.Plants (Basel, Switzerland) · 2019Review
- Mitochondrial Iron Transporters (MIT1 and MIT2) Are Essential for Iron Homeostasis and Embryogenesis inFrontiers in plant science · 2019Article
- Paralogs and mutants show that one DMA synthase functions in iron homeostasis in rice.Journal of experimental botany · 2017Article
- Regulating Subcellular Metal Homeostasis: The Key to Crop Improvement.Frontiers in plant science · 2016Review
- Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress.Frontiers in plant science · 2016Article
- Impairment of Respiratory Chain under Nutrient Deficiency in Plants: Does it Play a Role in the Regulation of Iron and Sulfur Responsive Genes?Frontiers in plant science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron (Fe) is an essential micronutrient for plant growth and development, and its reduced bioavailability strongly impairs mitochondrial functionality. In this work, the metabolic adjustment in the rice (Oryza sativa) mitochondrial Fe transporter knockdown mutant (mit-2) was analysed. Biochemical characterization of purified mitochondria from rice roots showed alteration in the respiratory chain of mit-2 compared with wild-type (WT) plants. In particular, proteins belonging to the type II alternative NAD(P)H dehydrogenases accumulated strongly in mit-2 plants, indicating that alternative pathways were activated to keep the respiratory chain working. Additionally, large-scale changes in the transcriptome and metabolome were observed in mit-2 rice plants. In particular, a strong alteration (up-/down-regulation) in the expression of genes encoding enzymes of both primary and secondary metabolism was found in mutant plants. This was reflected by changes in the metabolic profiles in both roots and shoots of mit-2 plants. Significant alterations in the levels of amino acids belonging to the aspartic acid-related pathways (aspartic acid, lysine, and threonine in roots, and aspartic acid and ornithine in shoots) were found that are strictly connected to the Krebs cycle. Furthermore, some metabolites (e.g. pyruvic acid, fumaric acid, ornithine, and oligosaccharides of the raffinose family) accumulated only in the shoot of mit-2 plants, indicating possible hypoxic responses. These findings suggest that the induction of local Fe deficiency in the mitochondrial compartment of mit-2 plants differentially affects the transcript as well as the metabolic profiles in root and shoot tissues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.