Evidence map›Paper›PMID 26685127›Full record

ArticleMolecular & cellular proteomics : MCP2016

Identification of the Post-translational Modifications Present in Centromeric Chromatin.

Aaron O Bailey, Tanya Panchenko, Jeffrey Shabanowitz, Stephanie M Lehman, Dina L Bai, Donald F Hunt, Ben E Black, Daniel R Foltz

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Article
  3. Preserving centromere identity: right amounts of CENP-A at the right place and time.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025
    Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Epigenetic regulation of centromere function.Cellular and molecular life sciences : CMLS · 2020
    Review
  15. One-Pot Quantitative Top- and Middle-Down Analysis of GluC-Digested Histone H4.Journal of the American Society for Mass Spectrometry · 2019
    Article
  16. Review
  17. Review
  18. Article
  19. Alpha satellite DNA biology: finding function in the recesses of the genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Aaron O BaileyFrom the ‡Department of Cell Biology, University of Virginia, Charlottesville, Virginia, 22908;
Tanya Panchenko§Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104-6059;
Jeffrey Shabanowitz¶Department of Chemistry, University of Virginia, Charlottesville, Virginia, 22908;
Stephanie M Lehman¶Department of Chemistry, University of Virginia, Charlottesville, Virginia, 22908;
Dina L Bai¶Department of Chemistry, University of Virginia, Charlottesville, Virginia, 22908;
Donald F Hunt¶Department of Chemistry, University of Virginia, Charlottesville, Virginia, 22908;
Ben E Black§Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104-6059; dfoltz@northwestern.edu blackbe@mail.med.upe.
Daniel R FoltzFrom the ‡Department of Cell Biology, University of Virginia, Charlottesville, Virginia, 22908; ‖Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, 22908; **Department of Biochemistry and Molecular Genetics, Northwestern University, Feinberg School of Medicine, Chicago Illinois 60611 dfoltz@northwestern.edu blackbe@mail.med.upe.
University of Virginia · USUniversity of Pennsylvania · USNorthwestern University · US

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Daniela E Matei · 1993 to 2026
$153.9M
PROTEIN SEQUENCING BY TANDEM MASS SPECTROMETRYR01GM037537 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI HUNT, DONALD F · 1987 to 2023
$17.8M
BIOTECHNOLOGY TRAINING PROGRAMT32GM008715 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI LAURIE, GORDON WILLIAM · 2000 to 2019
$6.6M
STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008275 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI WAND, A. JOSHUA · 1988 to 2018
$6.0M
Centromere Identity and FunctionR01GM082989 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI BLACK, BEN E. · 2008 to 2018
$3.6M
Assembly and epigenetic inheritance of the human centromereR01GM111907 · NIGMS · UNIVERSITY OF VIRGINIA · PI FOLTZ, DANIEL RICHARD · 2014 to 2022
$2.8M
NCI NIH HHS P30 CA060553NIGMS NIH HHS GM08275NIGMS NIH HHS R01 GM037537NIGMS NIH HHS R01 GM082989NIGMS NIH HHS R01 GM111907NIGMS NIH HHS T32 GM008275NIGMS NIH HHS T32 GM008715NIGMS NIH HHS T32 GM08715 T32
6 · The paper itself

Abstract

The centromere is the locus on the chromosome that acts as the essential connection point between the chromosome and the mitotic spindle. A histone H3 variant, CENP-A, defines the location of the centromere, but centromeric chromatin consists of a mixture of both CENP-A-containing and H3-containing nucleosomes. We report a surprisingly uniform pattern of primarily monomethylation on lysine 20 of histone H4 present in short polynucleosomes mixtures of CENP-A and H3 nucleosomes isolated from functional centromeres. Canonical H3 is not a component of CENP-A-containing nucleosomes at centromeres, so the H3 we copurify from these preparations comes exclusively from adjacent nucleosomes. We find that CENP-A-proximal H3 nucleosomes are not uniformly modified but contain a complex set of PTMs. Dually modified K9me2-K27me2 H3 nucleosomes are observed at the centromere. Side-chain acetylation of both histone H3 and histone H4 is low at the centromere. Prior to assembly at centromeres, newly expressed CENP-A is sequestered for a large portion of the cell cycle (late S-phase, G2, and most of mitosis) in a complex that contains its partner, H4, and its chaperone, HJURP. In contrast to chromatin associated centromeric histone H4, we show that prenucleosomal CENP-A-associated histone H4 lacks K20 methylation and contains side-chain and α-amino acetylation. We show HJURP displays a complex set of serine phosphorylation that may potentially regulate the deposition of CENP-A. Taken together, our findings provide key information regarding some of the key components of functional centromeric chromatin.

Indexed as

Protein Processing, Post-TranslationalAutoantigensCell CycleCentromereCentromere Protein AChromosomal Proteins, Non-HistoneDNA-Binding ProteinsHeLa CellsHistonesHumansLysineMethylationNucleosomesSerineSpectrometry, Mass, Electrospray IonizationAutoantigensCENPA protein, humanCentromere Protein AChromosomal Proteins, Non-HistoneDNA-Binding ProteinsHistonesHJURP protein, humanLysineNucleosomesSerine

Identifiers

PMID26685127
PMCPMC4813710
OpenAlexW2244029933

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.