ArticlePlant physiology2016
Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis.
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 37 citations in OpenAlex.
- COST1 stimulates RHD3 GTPase activity to maintain ER morphology and plant growth inScience advances · 2026Article
- Plasmodium SEY1 is a novel druggable target that contributes to imidazolopiperazine mechanism of action.Research square · 2024Article
- Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Characterization of intracellular membrane structures derived from a massive expansion of endoplasmic reticulum (ER) membrane due to synthetic ER-membrane-resident polyproteins.Journal of experimental botany · 2024Article
- Reconstitution of human atlastin fusion activity reveals autoinhibition by the C terminus.The Journal of cell biology · 2022Article
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- ROOT HAIR DEFECTIVE3 Is a Receptor for Selective Autophagy of the Endoplasmic Reticulum inFrontiers in plant science · 2022Article
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- The hypervariable region of atlastin-1 is a site for intrinsic and extrinsic regulation.The Journal of cell biology · 2021Article
- Maintaining the structural and functional homeostasis of the plant endoplasmic reticulum.Developmental cell · 2021Review
- Geometry and cellular function of organelle membrane interfaces.Plant physiology · 2021Review
- IntEResting structures: formation and applications of organized smooth endoplasmic reticulum in plant cells.Plant physiology · 2021Article
- The Sterol Trafficking Pathway inFrontiers in plant science · 2021Article
- The plant endoplasmic reticulum: an organized chaos of tubules and sheets with multiple functions.Journal of microscopy · 2020Review
- Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochromePlant signaling & behavior · 2020Article
- The Dynamin-Like GTPase FgSey1 Plays a Critical Role in Fungal Development and Virulence in Fusarium graminearum.Applied and environmental microbiology · 2020Article
- HIGH STEROL ESTER 1 is a key factor in plant sterol homeostasis.Nature plants · 2019Article
- Efficient ER Fusion Requires a Dimerization and a C-Terminal Tail Mediated Membrane Anchoring of RHD3.Plant physiology · 2018Article
- Advances in Plant ER Architecture and Dynamics.Plant physiology · 2018Review
- Modeling Endoplasmic Reticulum Network Maintenance in a Plant Cell.Biophysical journal · 2017Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Haruko UedaGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Etsuo YokotaGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.) yokota@sci.u-hyogo.ac.jp ihnishi@gr.bot.kyoto-u.ac.jp.
Keiko KuwataGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Natsumaro KutsunaGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Shoji ManoGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Tomoo ShimadaGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Kentaro TamuraGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Giovanni StefanoGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).ORCID http://orcid.org/0000-0002-2744-0052
Yoichiro FukaoGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Federica BrandizziGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Teruo ShimmenGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Mikio NishimuraGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.).
Ikuko Hara-NishimuraGraduate School of Science, Kyoto University, Kyoto 606-8502, Japan (H.U., To.S., K.T., I.H.-N.); Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan (E.Y., Te.S.); Institute of Transformative Bio-Molecules, Nagoya University, Nagoya 464-8601, Japan (K.K.); Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan (N.K.); Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan (S.M., M.N.); MSU-DOE Plant Research Laboratory and Department of Plant Biology, Michigan State University, Michigan 48824 (G.S., F.B.); and Department of Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan (Y.F.) yokota@sci.u-hyogo.ac.jp ihnishi@gr.bot.kyoto-u.ac.jp.ORCID http://orcid.org/0000-0001-8814-1593
Kyoto University · JPNational Institute for Basic Biology · JPUniversity of Hyogo · JPRitsumeikan University · JP
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endoplasmic reticulum (ER) consists of dynamically changing tubules and cisternae. In animals and yeast, homotypic ER membrane fusion is mediated by fusogens (atlastin and Sey1p, respectively) that are membrane-associated dynamin-like GTPases. In Arabidopsis (Arabidopsis thaliana), another dynamin-like GTPase, ROOT HAIR DEFECTIVE3 (RHD3), has been proposed as an ER membrane fusogen, but direct evidence is lacking. Here, we show that RHD3 has an ER membrane fusion activity that is enhanced by phosphorylation of its C terminus. The ER network was RHD3-dependently reconstituted from the cytosol and microsome fraction of tobacco (Nicotiana tabacum) cultured cells by exogenously adding GTP, ATP, and F-actin. We next established an in vitro assay system of ER tubule formation with Arabidopsis ER vesicles, in which addition of GTP caused ER sac formation from the ER vesicles. Subsequent application of a shearing force to this system triggered the formation of tubules from the ER sacs in an RHD-dependent manner. Unexpectedly, in the absence of a shearing force, Ser/Thr kinase treatment triggered RHD3-dependent tubule formation. Mass spectrometry showed that RHD3 was phosphorylated at multiple Ser and Thr residues in the C terminus. An antibody against the RHD3 C-terminal peptide abolished kinase-triggered tubule formation. When the Ser cluster was deleted or when the Ser residues were replaced with Ala residues, kinase treatment had no effect on tubule formation. Kinase treatment induced the oligomerization of RHD3. Neither phosphorylation-dependent modulation of membrane fusion nor oligomerization has been reported for atlastin or Sey1p. Taken together, we propose that phosphorylation-stimulated oligomerization of RHD3 enhances ER membrane fusion to form the ER network.
Indexed as
Membrane FusionAmino Acid SequenceArabidopsisArabidopsis ProteinsBiological AssayEndoplasmic ReticulumGTP-Binding ProteinsGuanosine TriphosphateIntracellular MembranesMolecular Sequence DataPhosphopeptidesPhosphorylationProtein KinasesProtein MultimerizationSerineArabidopsis ProteinsGTP-Binding ProteinsGuanosine TriphosphatePhosphopeptidesProtein KinasesRHD3 protein, ArabidopsisSerine
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