ArticlePLoS genetics2015
Seed Dormancy in Arabidopsis Requires Self-Binding Ability of DOG1 Protein and the Presence of Multiple Isoforms Generated by Alternative Splicing.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- DOG1-Mediated Priming Followed by Environmentally Tunable Plasticity: A Two-Phase Model for Dormancy Establishment inProteomes · 2026Article
- Genome-Wide Identification and Expression Profiling of theInternational journal of molecular sciences · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Genome-wide identification and analysis of the Delay Of Germination 1 (DOG1) gene family in Brassica napus and its potential role in Manganese (Mn) stress response.BMC plant biology · 2026Article
- Re-examining potato dormancy in the context of commercial tuber storage.Journal of experimental botany · 2026Review
- Translating research on seed dormancy and germination from Arabidopsis to temperate cereals to control pre-harvest sprouting.Frontiers in plant science · 2026Review
- UBA2A regulates seed dormancy and the stability of chromatin-retained DOG1 messenger RNA.Journal of integrative plant biology · 2025Article
- Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy.Nature communications · 2025Article
- Identification of tomato F-box proteins functioning in phenylpropanoid metabolism.Plant molecular biology · 2024Article
- Importance of pre-mRNA splicing and its study tools in plants.Advanced biotechnology · 2024Review
- Alternative Splicing during Fiber Development inInternational journal of molecular sciences · 2023Article
- Catsnap: a user-friendly algorithm for determining the conservation of protein variants reveals extensive parallelisms in the evolution of alternative splicing.The New phytologist · 2023Article
- The pattern of alternative splicing and DNA methylation alteration and their interaction in linseed (Linum usitatissimum L.) response to repeated drought stresses.Biological research · 2023Article
- Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1.Frontiers in plant science · 2023Article
- Alternative splicing in ABA signaling during seed germination.Frontiers in plant science · 2023Review
- Current overview on the genetic basis of key genes involved in soybean domestication.aBIOTECH · 2022Review
- How alternative splicing changes the properties of plant proteins.Quantitative plant biology · 2022Review
- Article
- Post-transcriptional regulation of seed dormancy and germination: Current understanding and future directions.Plant communications · 2021Review
- Normal, novel or none: versatile regulation from alternative splicing.Plant signaling & behavior · 2021Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis protein DELAY OF GERMINATION 1 (DOG1) is a key regulator of seed dormancy, which is a life history trait that determines the timing of seedling emergence. The amount of DOG1 protein in freshly harvested seeds determines their dormancy level. DOG1 has been identified as a major dormancy QTL and variation in DOG1 transcript levels between accessions contributes to natural variation for seed dormancy. The DOG1 gene is alternatively spliced. Alternative splicing increases the transcriptome and proteome diversity in higher eukaryotes by producing transcripts that encode for proteins with altered or lost function. It can also generate tissue specific transcripts or affect mRNA stability. Here we suggest a different role for alternative splicing of the DOG1 gene. DOG1 produces five transcript variants encoding three protein isoforms. Transgenic dog1 mutant seeds expressing single DOG1 transcript variants from the endogenous DOG1 promoter did not complement because they were non-dormant and lacked DOG1 protein. However, transgenic plants overexpressing single DOG1 variants from the 35S promoter could accumulate protein and showed complementation. Simultaneous expression of two or more DOG1 transcript variants from the endogenous DOG1 promoter also led to increased dormancy levels and accumulation of DOG1 protein. This suggests that single isoforms are functional, but require the presence of additional isoforms to prevent protein degradation. Subsequently, we found that the DOG1 protein can bind to itself and that this binding is required for DOG1 function but not for protein accumulation. Natural variation for DOG1 binding efficiency was observed among Arabidopsis accessions and contributes to variation in seed dormancy.
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