ArticleCell stress & chaperones2016
Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression.
Article in Cell stress & chaperones, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 47 citations in OpenAlex.
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- Chronic Heat Stress Caused Lipid Metabolism Disorder and Tissue Injury in the Liver ofAntioxidants (Basel, Switzerland) · 2025Article
- State of thermal tolerance in an endangered himalayan fish Tor putitora revealed by expression modulation in environmental stress related genes.Scientific reports · 2025Article
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- Seasonal variation in the expression pattern of heat shock protein 70 and 90 in Common carp (Veterinary research forum : an international quarterly journal · 2023Article
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- Molecular cloning, expression HSP70 and its response to bacterial challenge and heat stress in Microptenus salmoides.Fish physiology and biochemistry · 2020Article
- Temperature training improves transcriptional homeostasis after heat shock in juvenile Atlantic sturgeon (Acipenser oxyrinchus).Fish physiology and biochemistry · 2020Article
- Gill transcriptomes reveal expression changes of genes related with immune and ion transport under salinity stress in silvery pomfret (Pampus argenteus).Fish physiology and biochemistry · 2020Article
- Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is More Stressful to the SilversideFrontiers in genetics · 2018Article
- Effects of different rearing temperatures on muscle development and stress response in the early larval stages of Acipenser baerii.European journal of histochemistry : EJH · 2017Article
- Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti.Cell stress & chaperones · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genes encoding HSP70 and HSP90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary DNA (cDNA) ends (RACE). HSP70 (GenBank accession no. KP050541) and HSP90 (GenBank accession no. KP050542) cDNAs were composed of 2275 and 2718 bp and encoded polypeptides of 650 and 725 amino acids, respectively. Basic Local Alignment Search Tool (BLAST) analysis showed that HSP70 and HSP90 of kaluga shared high identities with those of Acipenser ruthenus, Acipenser schrenckii, and Acipenser baerii (98-99 %). Fluorescent real-time RT-PCR under unstressed conditions revealed that HSP70 and HSP90 were expressed in 11 different tissues of kaluga. Messenger RNA (mRNA) expressions of both HSP70 and HSP90 were highest in the intestine and lowest in the muscle. In addition, the patterns of mRNA expression of HSP70 and HSP90 were similar, although the level of expression was more in HSP90 than in HSP70 (P < 0.05).We also analyzed patterns of HSP70 and HSP90 expression in the muscle, gill, and liver of kaluga under different combinations of temperature and salinity stress, including temperatures of 4,10, 25, and 28 °C at 0 ppt salinity, and salinities of 10, 20, 30, and 40 ppt at 16 °C, where 16 °C at 0 ppt (parts per thousand) served as the control. We found that levels of mRNA expression of both HSP70 and HSP90 were highest at 4 °C in the muscle, gill, and liver and changed little with salinity stress. These results increase understanding of the mechanisms of stress response of cold freshwater fish.
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