ArticleLife sciences2016
Two-photon imaging of endothelin-1-mediated intracellular Ca(2+) handling in smooth muscle cells of rat renal resistance arteries.
Article in Life sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 5 citations in OpenAlex.
- Molecular and cellular basis of praziquantel action in the cardiovascular system.American journal of physiology. Cell physiology · 2023Article
- Renal vascular TRP channels.Current research in physiology · 2021Article
- Human genotyping and an experimental model reveal NPR-C as a possible contributor to morbidity in coarctation of the aorta.Physiological genomics · 2019Article
- Endothelin receptor A and p66Shc regulate spontaneous CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- p66Shc regulates renal vascular tone in hypertension-induced nephropathy.The Journal of clinical investigation · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
aimsEndothelin-1 (ET-1) is a potent vasoconstrictor which regulates the physiology of cardiorenal system. The aim of this study was to evaluate ET-1-mediated elevation of intracellular Ca(2+) in smooth muscle cells (SMC) of renal resistance arteries. MAIN
methodsIn in vitro studies of primary SMC, which were isolated from rat renal microvessels, the levels of intracellular Ca(2+) were calculated from the ratio of emissions at 340 and 380nm after loading cells with Fura 2-AM dye. In ex vivo studies we used two-photon imaging of renal resistance arteries excised from rat kidneys and loaded with fluorescent Ca(2+) indicator Fluo-4 AM. KEY
findingsThe two-photon imaging demonstrates that treatment of isolated rat renal resistance arteries with ET-1 causes a rapid increase of intracellular Ca(2+) concentration in smooth muscle vasculature of these vessels. These ex vivo observations are in accordance with in vitro findings indicating that ET-1 mediates activation of TRPC channels and increases the level of intracellular Ca(2+) in cultured SMC to 510±83nM. SIGNIFICANCE: ET-1-mediated elevation of intracellular Ca(2+) is strongly linked to renal microvascular contraction and is crucial for ET-1-induced contraction of SMC. The two-photon imaging of intracellular Ca(2+) in intact SMC of rat renal resistance arteries is a powerful technique which allows the detailed ex vivo analysis of intracellular Ca(2+) handling by ET-1, an important player in hypertension-related kidney diseases.
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