ArticleApplied and environmental microbiology2016
Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 94 citations in OpenAlex.
- Impact of carbon and nitrogen source variation on B-vitamin accumulation inBiochemistry and biophysics reports · 2026Article
- Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production inSynthetic and systems biotechnology · 2026Article
- Xylose Metabolism Perturbation in Yarrowia lipolytica for Efficient Succinic Acid Bioproduction from Lignocellulosic Biomass.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production.Microbial biotechnology · 2025Article
- Review
- Genome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica.Biotechnology for biofuels and bioproducts · 2024Article
- Proteomes reveal metabolic capabilities ofmSystems · 2023Article
- Installing xylose assimilation and cellodextrin phosphorolysis pathways in obese Yarrowia lipolytica facilitates cost-effective lipid production from lignocellulosic hydrolysates.Biotechnology for biofuels and bioproducts · 2023Article
- Codon Optimization Improves the Prediction of Xylose Metabolism from Gene Content in Budding Yeasts.Molecular biology and evolution · 2023Article
- Lipid production from lignocellulosic biomass using an engineered Yarrowia lipolytica strain.Microbial cell factories · 2022Article
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Insights into the glycerol transport of Yarrowia lipolytica.Yeast (Chichester, England) · 2022Article
- Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineeredFrontiers in microbiology · 2022Article
- Article
- Review
- Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin.Applied microbiology and biotechnology · 2021Review
- Methods to Activate and Elucidate Complex Endogenous Sugar Metabolism in Yarrowia lipolytica.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Yeast-Based Biosynthesis of Natural Products From Xylose.Frontiers in bioengineering and biotechnology · 2021Review
- Disruption of theFrontiers in microbiology · 2021Article
- Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.MicrobiologyOpen · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The oleaginous yeast Yarrowia lipolytica is an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism of Y. lipolytica for using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and elucidated the native sugar metabolism in Y. lipolytica for cell growth on xylose and cellobiose as well as their mixtures with glucose through comprehensive metabolic and transcriptomic analyses. We identified 7 putative glucose-specific transporters, 16 putative xylose-specific transporters, and 4 putative cellobiose-specific transporters that are transcriptionally upregulated for growth on respective single sugars. Y. lipolytica is capable of using xylose as a carbon source, but xylose dehydrogenase is the key bottleneck of xylose assimilation and is transcriptionally repressed by glucose. Y. lipolytica has a set of 5 extracellular and 6 intracellular β-glucosidases and is capable of assimilating cellobiose via extra- and intracellular mechanisms, the latter being dominant for growth on cellobiose as a sole carbon source. Strikingly, Y. lipolytica exhibited enhanced sugar utilization for growth in mixed sugars, with strong carbon catabolite activation for growth on the mixture of xylose and cellobiose and with mild carbon catabolite repression of glucose on xylose and cellobiose. The results of this study shed light on fundamental understanding of the complex native sugar metabolism of Y. lipolytica and will help guide inverse metabolic engineering of Y. lipolytica for enhanced conversion of biomass-derived fermentable sugars to chemicals and fuels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.