ArticleBiophysical journal2015
ATP Consumption of Eukaryotic Flagella Measured at a Single-Cell Level.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 91 citations in OpenAlex.
- Embodied behavioural complexity in a ciliated microorganism.Nature communications · 2026Article
- Acceleration and Light-Induced Changes in Cytosolic cAMP Concentration inBiomolecules · 2026Article
- Crosslinked F-actin networks regulate load-dependent energy conversion.Communications biology · 2026Article
- Costs and benefits of phytoplankton motility.ArXiv · 2025Article
- In situ structure of the mouse sperm central apparatus reveals mechanistic insights into asthenozoospermia.Cell research · 2025Article
- Structural Rearrangement of the Olfactory Epithelium in Male Baikal Yellowfin Sculpins Across the Reproductive Period.Biology · 2025Article
- Article
- Active fluctuations of axoneme oscillations scale with number of dynein motors.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model.Nature communications · 2024Article
- Intraflagellar transport speed is sensitive to genetic and mechanical perturbations to flagellar beating.The Journal of cell biology · 2024Article
- Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination.Nature communications · 2024Article
- F-actin architecture determines the conversion of chemical energy into mechanical work.Nature communications · 2024Article
- Mitochondrial uncoupling proteins protect human airway epithelial ciliated cells from oxidative damage.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Pyruvate modulation of redox potential controls mouse sperm motility.Developmental cell · 2024Article
- How much (ATP) does it cost to build a trypanosome? A theoretical study on the quantity of ATP needed to maintain and duplicate a bloodstream-form Trypanosoma brucei cell.PLoS pathogens · 2023Article
- Flagellum-driven cargoes: Influence of cargo size and the flagellum-cargo attachment geometry.PloS one · 2023Article
- Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions.International journal of molecular sciences · 2022Article
- Self-mixing in microtubule-kinesin active fluid from nonuniform to uniform distribution of activity.Nature communications · 2022Article
- Eukaryotic gene regulation at equilibrium, or non?Current opinion in systems biology · 2022Article
- Flagellar energy costs across the tree of life.eLife · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The motility of cilia and flagella is driven by thousands of dynein motors that hydrolyze adenosine triphosphate (ATP). Despite decades of genetic, biochemical, structural, and biophysical studies, some aspects of ciliary motility remain elusive, such as the regulation of beating patterns and the energetic efficiency of these nanomachines. In this study, we introduce an experimental method to measure ATP consumption of actively beating axonemes on a single-cell level. We encapsulated individual sea urchin sperm with demembranated flagellum inside water-in-oil emulsion droplets and measured the axoneme's ATP consumption by monitoring fluorescence intensity of a fluorophore-coupled reporter system for ATP turnover in the droplet. Concomitant phase contrast imaging allowed us to extract a linear dependence between the ATP consumption rate and the flagellar beating frequency, with ∼2.3 × 10(5) ATP molecules consumed per beat of a demembranated flagellum. Increasing the viscosity of the aqueous medium led to modified beating waveforms of the axonemes and to higher energy consumption per beat cycle. Our single-cell experimental platform provides both new insights, to our knowledge, into the beating mechanism of flagella and a powerful tool for future studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.