ArticleBiophysical journal2015
Mechanics of Biomimetic Liposomes Encapsulating an Actin Shell.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Balanced contractility and adhesion drive polarization in a minimal elastic actomyosin network.PLoS computational biology · 2026Article
- Benefits and challenges of reconstituting the actin cortex.Cytoskeleton (Hoboken, N.J.) · 2024Review
- Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model.Communications biology · 2024Article
- Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes.Physiology (Bethesda, Md.) · 2024Review
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex.Communications biology · 2023Article
- Bioinspired Membrane Interfaces: Controlling Actomyosin Architecture and Contractility.ACS applied materials & interfaces · 2023Article
- Compressibility and porosity modulate the mechanical properties of giant gas vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Unveiling the physics underlying symmetry breaking of the actin cytoskeleton: An artificial cell-based approach.Biophysics and physicobiology · 2023Article
- Scratching beyond the surface - minimal actin assemblies as tools to elucidate mechanical reinforcement and shape change.Emerging topics in life sciences · 2022Article
- Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks.Biochemical Society transactions · 2022Review
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- Article
- Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.Communications biology · 2021Article
- Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles.ACS synthetic biology · 2021Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- A self-organized synthetic morphogenic liposome responds with shape changes to local light cues.Nature communications · 2021Article
- Actin modulates shape and mechanics of tubular membranes.Science advances · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-shape changes are insured by a thin, dynamic, cortical layer of cytoskeleton underneath the plasma membrane. How this thin cortical structure impacts the mechanical properties of the whole cell is not fully understood. Here, we study the mechanics of liposomes or giant unilamellar vesicles, when a biomimetic actin cortex is grown at the inner layer of the lipid membrane via actin-nucleation-promoting factors. Using a hydrodynamic tube-pulling technique, we show that tube dynamics is clearly affected by the presence of an actin shell anchored to the lipid bilayer. The same force pulls much shorter tubes in the presence of the actin shell compared to bare membranes. However, in both cases, we observe that the dynamics of tube extrusion has two distinct features characteristic of viscoelastic materials: rapid elastic elongation, followed by a slower elongation phase at a constant rate. We interpret the initial elastic regime by an increase of membrane tension due to the loss of lipids into the tube. Tube length is considerably shorter for cortex liposomes at comparable pulling forces, resulting in a higher spring constant. The presence of the actin shell seems to restrict lipid mobility, as is observed in the corral effect in cells. The viscous regime for bare liposomes corresponds to a leakout of the internal liquid at constant membrane tension. The presence of the actin shell leads to a larger friction coefficient. As the tube is pulled from a patchy surface, membrane tension increases locally, leading to a Marangoni flow of lipids. As a conclusion, the presence of an actin shell is revealed by its action that alters membrane mechanics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.