ArticleNucleic acids research2016
Locking-to-unlocking system is an efficient strategy to design DNA/silver nanoclusters (AgNCs) probe for human miRNAs.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Identification of specific miRNAs in early-stage mung bean (Horticulture research · 2026Article
- The spatial organization of trace silver atoms on a DNA template.RSC advances · 2020Review
- Preparation and characterization of solid DNA silver nanoclusters with superior aerobic and thermal stability.RSC advances · 2019Article
- Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence.Nanomaterials (Basel, Switzerland) · 2019Article
- Advanced microRNA-based cancer diagnostics using amplified time-gated FRET.Chemical science · 2018Article
- Colorimetric and energy transfer based fluorometric turn-on method for determination of microRNA using silver nanoclusters and gold nanoparticles.Mikrochimica acta · 2018Article
- FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs.Nucleic acids research · 2017Article
- MicroRNA Biomarkers in Neurodegenerative Diseases and Emerging Nano-Sensors Technology.Journal of movement disorders · 2017Review
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Authors and funding
9 authors.
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Abstract
MicroRNAs (miRNAs), small non-coding RNA molecules, are important biomarkers for research and medical purposes. Here, we describe the development of a fast and simple method using highly fluorescent oligonucleotide-silver nanocluster probes (DNA/AgNCs) to efficiently detect specific miRNAs. Due to the great sequence diversity of miRNAs in humans and other organisms, a uniform strategy for miRNA detection is attractive. The concept presented is an oligonucleotide-based locking-to-unlocking system that can be endowed with miRNA complementarity while maintaining the same secondary structure. The locking-to-unlocking system is based on fold-back anchored DNA templates that consist of a cytosine-rich loop for AgNCs stabilization, an miRNA recognition site and an overlap region for hairpin stabilization. When an miRNA is recognized, fluorescence in the visible region is specifically extinguished in a concentration-dependent manner. Here, the exact composition of the fold-back anchor for the locking-to-unlocking system has been systematically optimized, balancing propensity for loop-structure formation, encapsulation of emissive AgNCs and target sensitivity. It is demonstrated that the applied strategy successfully can detect a number of cancer related miRNAs in RNA extracts from human cancer cell lines.
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