Evidence map›Paper›PMID 26681515›Full record

ArticleG3 (Bethesda, Md.)2015

MicroRNA Maturation and MicroRNA Target Gene Expression Regulation Are Severely Disrupted in Soybean dicer-like1 Double Mutants.

Shaun J Curtin, Jean-Michel Michno, Benjamin W Campbell, Javier Gil-Humanes, Sandra M Mathioni, Reza Hammond, Juan J Gutierrez-Gonzalez, Ryan C Donohue, Michael B Kantar, Andrew L Eamens and 3 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
5.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. MicroRNAs Responding to Space Radiation.International journal of molecular sciences · 2020
    Review
  8. Article
  9. Article
  10. Mutations inThe Plant cell · 2017
    Article
  11. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Shaun J CurtinDepartment of Plant Pathology, University of Minnesota, St. Paul, Minnesota, 55108 curti242@umn.edu rstupar@umn.edu.ORCID http://orcid.org/0000-0002-9528-3335
Jean-Michel MichnoDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108.
Benjamin W CampbellDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108.
Javier Gil-HumanesDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455 Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455.
Sandra M MathioniDepartment of Plant and Soil Sciences, University of Delaware, Newark, Delaware 19711.
Reza HammondDepartment of Plant and Soil Sciences, University of Delaware, Newark, Delaware 19711.
Juan J Gutierrez-GonzalezDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108.
Ryan C DonohueDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108.
Michael B KantarDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108.
Andrew L EamensSchool of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales 2308, Australia.
Blake C MeyersDepartment of Plant and Soil Sciences, University of Delaware, Newark, Delaware 19711.
Daniel F VoytasDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455 Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455.
Robert M StuparDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, 55108 curti242@umn.edu rstupar@umn.edu.
University of Minnesota · USUniversity of Delaware · USUniversity of Newcastle Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Small nonprotein-coding microRNAs (miRNAs) are present in most eukaryotes and are central effectors of RNA silencing-mediated mechanisms for gene expression regulation. In plants, DICER-LIKE1 (DCL1) is the founding member of a highly conserved family of RNase III-like endonucleases that function as core machinery proteins to process hairpin-like precursor transcripts into mature miRNAs, small regulatory RNAs, 21-22 nucleotides in length. Zinc finger nucleases (ZFNs) were used to generate single and double-mutants of putative soybean DCL1 homologs, DCL1a and DCL1b, to confirm their functional role(s) in the soybean miRNA pathway. Neither DCL1 single mutant, dcl1a or dcl1b plants, exhibited a pronounced morphological or molecular phenotype. However, the dcl1a/dcl1b double mutant expressed a strong morphological phenotype, characterized by reduced seed size and aborted seedling development, in addition to defective miRNA precursor transcript processing efficiency and deregulated miRNA target gene expression. Together, these findings indicate that the two soybean DCL1 paralogs, DCL1a and DCL1b, largely play functionally redundant roles in the miRNA pathway and are essential for normal plant development.

Indexed as

Gene Expression Regulation, PlantMutationRNA InterferenceRNA StabilityAllelesBase SequenceCluster AnalysisGene Expression ProfilingGlycine maxMicroRNAsMutagenesis, Site-DirectedPhenotypeProtein BindingRibonuclease IIIRibonucleasesRNA, MessengerMicroRNAsRibonuclease IIIRibonucleasesRNA, Messengerdicer-likegenome engineeringmiRNAsoybeanZFN

Identifiers

PMID26681515
PMCPMC4751560
OpenAlexW2284515829

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.