ArticleBMC developmental biology2015
Transcriptome profiling of bovine inner cell mass and trophectoderm derived from in vivo generated blastocysts.
Article in BMC developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Cross-species meta-analysis of transcriptome changes during the morula-to-blastocyst transition: metabolic and physiological changes take center stage.American journal of physiology. Cell physiology · 2021Pooled it
- Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis.Frontiers in genetics · 2024Article
- Adaptative response to changes in pyruvate metabolism on the epigenetic landscapes and transcriptomics of bovine embryos.Scientific reports · 2023Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero.Reproduction & fertility · 2023Article
- Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes.Frontiers in endocrinology · 2023Article
- Effects of Extra-Long-Acting Recombinant Bovine FSH (bscrFSH) on Cattle Superovulation.Animals : an open access journal from MDPI · 2022Article
- The global gene expression outline of the bovine blastocyst: reflector of environmental conditions and predictor of developmental capacity.BMC genomics · 2021Article
- Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle.International journal of molecular sciences · 2021Review
- PGE2 Supplementation of Oocyte Culture Media Improves the Developmental and Cryotolerance Performance of Bovine Blastocysts Derived From a Serum-FreeFrontiers in cell and developmental biology · 2021Article
- Notch signaling in mouse blastocyst development and hatching.BMC developmental biology · 2020Article
- Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos.The Journal of reproduction and development · 2020Article
- Can trophectoderm RNA analysis predict human blastocyst competency?Systems biology in reproductive medicine · 2019Article
- Dynamics of The Expression of Pluripotency and Lineage Specific Genes in The Pre and Peri-Implantation Goat Embryo.Cell journal · 2019Article
- Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts.Biology open · 2019Article
- Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos.Journal of assisted reproduction and genetics · 2019Article
- Interleukin-6 increases inner cell mass numbers in bovine embryos.BMC developmental biology · 2019Article
- Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP.PloS one · 2019Article
- Transcriptome Analysis of Immune Response of mIgMFrontiers in immunology · 2019Article
- Specification of trophoblast from embryonic stem cells exposed to BMP4.Biology of reproduction · 2018Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis study describes the generation and analysis of the transcriptional profile of bovine inner cell mass (ICM) and trophectoderm (TE), obtained from in vivo developed embryos by using a bovine-embryo specific array (EmbryoGENE) containing 37,238 probes.
resultsA total of 4,689 probes were differentially expressed between ICM and TE, among these, 2,380 and 2,309 probes were upregulated in ICM and TE tissues, respectively (P ≤ 0.01, FC ≥ 2.0, FDR: 2.0). Ontological classification of the genes predominantly expressed in ICM emerged a range of functional categories with a preponderance of genes involved in basal and developmental signaling pathways including P53, TGFβ, IL8, mTOR, integrin, ILK, and ELF2 signalings. Cross-referencing of microarray data with two available in vitro studies indicated a marked reduction in ICM vs. TE transcriptional difference following in vitro culture of bovine embryos. Moreover, a great majority of genes that were found to be misregulated following in vitro culture of bovine embryos were known genes involved in epigenetic regulation of pluripotency and cell differentiation including DNMT1, GADD45, CARM1, ELF5 HDAC8, CCNB1, KDM6A, PRDM9, CDX2, ARID3A, IL6, GADD45A, FGFR2, PPP2R2B, and SMARCA2. Cross-species referencing of microarray data revealed substantial divergence between bovine and mouse and human in signaling pathways involved in early lineage specification.
conclusionsThe transcriptional changes occur during ICM and TE lineages specification in bovine is greater than previously understood. Therefore, this array data establishes a standard to evaluate the in vitro imprint on the transcriptome and to hypothesize the cross-species differences that allow in vitro acquisition of pluripotent ICM in human and mice but hinder that process in bovine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.