ArticleScientific reports2015
Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Mechanisms of Red-Shifted Absorption in Biliverdin-Binding Proteins.Biochemistry · 2026Article
- Nonlinear optical properties of photosensory core modules of monomeric and dimeric bacterial phytochromes.Protein science : a publication of the Protein Society · 2025Article
- Multi-color fluorescence live-cell imaging in Dictyostelium discoideum.Cell structure and function · 2024Article
- Hormogonium Development and Motility in Filamentous Cyanobacteria.Applied and environmental microbiology · 2023Review
- Anaerobic fluorescent reporters for live imaging ofFrontiers in microbiology · 2023Article
- A Ubiquitously Conserved Cyanobacterial Protein Phosphatase Essential for High Light Tolerance in a Fast-Growing Cyanobacterium.Microbiology spectrum · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets.ACS synthetic biology · 2021Article
- Near-infrared and far-red genetically encoded indicators of neuronal activity.Journal of neuroscience methods · 2021Article
- GAF-CaMP3-sfGFP, An Enhanced Version of the Near-Infrared Genetically Encoded Positive Phytochrome-Based Calcium Indicator for the Visualization of Neuronal Activity.International journal of molecular sciences · 2020Article
- A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales.Nature communications · 2020Article
- Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.Current opinion in structural biology · 2019Review
- Near-Infrared Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial Phytochrome.International journal of molecular sciences · 2019Article
- A genetically encoded fluorescent temperature sensor derived from the photoactive Orange Carotenoid Protein.Scientific reports · 2019Article
- Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing.Nature communications · 2019Article
- Article
- De novo synthetic biliprotein design, assembly and excitation energy transfer.Journal of the Royal Society, Interface · 2018Article
- Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging.Biophysical journal · 2017Article
- Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes.International journal of molecular sciences · 2017Review
- Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies.Chemical science · 2017Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of near-infrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engineered R. palustris bacterial phytochrome into a single-domain NIR FP of 19.6 kDa, termed GAF-FP, which is 2-fold and 1.4-fold smaller than bacterial phytochrome-based NIR FPs and GFP-like proteins, respectively. Engineering of GAF-FP involved a substitution of 15% of its amino acids and a deletion of the knot structure. GAF-FP covalently binds two tetrapyrrole chromophores, biliverdin (BV) and phycocyanobilin (PCB). With the BV chromophore GAF-FP absorbs at 635 nm and fluoresces at 670 nm. With the PCB chromophore GAF-FP becomes blue-shifted and absorbs at 625 nm and fluoresces at 657 nm. The GAF-FP structure has a high tolerance to small peptide insertions. The small size of GAF-FP and its additional absorbance band in the violet range has allowed for designing a chimeric protein with Renilla luciferase. The chimera exhibits efficient non-radiative energy transfer from luciferase to GAF-FP, resulting in NIR bioluminescence. This study opens the way for engineering of small NIR FPs and NIR luciferases from bacterial phytochromes.
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