Evidence map›Paper›PMID 26678450›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2016

The integrated endoplasmic reticulum stress response in Leishmania amazonensis macrophage infection: the role of X-box binding protein 1 transcription factor.

Karina Luiza Dias-Teixeira, Teresa Cristina Calegari-Silva, Guilherme R R M dos Santos, José Vitorino Dos Santos, Carolina Lima, Jorge Mansur Medina, Bertal Huseyin Aktas, Ulisses G Lopes

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 31 citations in OpenAlex.

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  15. Article
  16. Type I interferons and endoplasmic reticulum stress in health and disease.International review of cell and molecular biology · 2020
    Review
  17. Review
  18. Article
  19. Emerging therapeutic targets for treatment of leishmaniasis.Expert opinion on therapeutic targets · 2018
    Review
  20. Frontiers in microbiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Karina Luiza Dias-Teixeira*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Teresa Cristina Calegari-Silva*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Guilherme R R M dos Santos*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
José Vitorino Dos Santos*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Carolina Lima*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Jorge Mansur Medina*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Bertal Huseyin Aktas*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Ulisses G Lopes*Laboratório de Parasitologia Molecular, Instituto de Biofísica Carlos Chagas Filho, and Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; and Hematology Laboratory for Translation, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA lopesu@biof.ufrj.br.
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro · BRUniversidade Federal do Rio de Janeiro · BRBrigham and Women's Hospital · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endoplasmic reticulum (ER) stress triggers the integrated ER-stress response (IERSR) that ensures cellular survival of ER stress and represents a primordial form of innate immunity. We investigated the role of IERSR duringLeishmania amazonensisinfection. Treatment of RAW 264.7 infected macrophages with the ER stress-inducing agent thapsigargin (TG; 1 μM) increasedL. amazonensisinfectivity in an IFN1-α receptor (IFNAR)-dependent manner. In Western blot assays, we showed thatL. amazonensisactivates the inositol-requiring enzyme (IRE1)/ X-box binding protein (XBP)-1-splicing arms of the IERSR in host cells. In chromatin immunoprecipitation (ChIP) assays, we showed an increased occupancy of enhancer and promoter sequences for theIfnbgene by XBP1 in infected RAW 264.7 cells. Knocking down XBP1 expression by transducing RAW 264.7 cells with the short hairpin XBP1 lentiviral vector significantly reduced the parasite proliferation associated with impaired translocation of phosphorylated IFN regulatory transcription factor (IRF)-3 to the nucleus and a decrease in IFN1-β expression. Knocking down XBP1 expression also increased NO concentration, as determined by Griess reaction and reduced the expression of antioxidant genes, such as heme oxygenase (HO)-1, that protect parasites from oxidative stress. We conclude thatL. amazonensisactivation of XBP1 plays a critical role in infection by protecting the parasites from oxidative stress and increasing IFN1-β expression.-Dias-Teixeira, K. L., Calegari-Silva, T. C., Dos Santos, G. R. R. M., Vitorino dos Santos, J., Lima, C., Medina, J. M., Aktas, B. H., Lopes, U. G. The integrated endoplasmic reticulum stress response inLeishmania amazonensismacrophage infection: the role of X-box binding protein 1 transcription factor.

Indexed as

Endoplasmic Reticulum StressAnimalsBlotting, WesternCell LineDNA-Binding ProteinsGene ExpressionHEK293 CellsHeme Oxygenase-1Host-Parasite InteractionsHumansInterferon-betaLeishmaniaMacrophagesMicePromoter Regions, GeneticProtein BindingDNA-Binding ProteinsHeme Oxygenase-1Interferon-betaReactive Oxygen SpeciesRegulatory Factor X Transcription FactorsThapsigarginTranscription FactorsX-Box Binding Protein 1XBP1 protein, humanXbp1 protein, mouseER stressIFN1-βoxidative stressthapsigargin IRE1XBP1

Identifiers

PMID26678450
PMCPMC7163978
OpenAlexW2219742936

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.