ArticleStem cell reports2015
FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal.
Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
115 citing papers in PubMed, 235 citations in OpenAlex.
- Development of serum-free cryomedia for slow-freezing of chicken primordial germ cells.The Journal of reproduction and development · 2026Article
- Short-term gonadal cultures are sufficient for germline transmission in a songbird.Stem cell reports · 2026Article
- Long-term rock dove (Columba livia) primordial germ cell culture: A tool towards avian conservation.The Journal of heredity · 2026Article
- Successful establishment of chicken primordial germ cell (PGC) lines from the endangered Slovakian indigenous chicken breed: the Oravka.Poultry science · 2026Article
- Derivation of embryonic stem cells across avian species.Nature biotechnology · 2026Article
- Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.Cellular and molecular life sciences : CMLS · 2026Article
- The chicken retrovirus-like geneiScience · 2026Article
- Review
- Transcriptional Profiling of Primordial Germ Cells During Chicken Embryonic Development.Veterinary sciences · 2026Article
- Deciphering interferon functions in avian influenza using receptor knockout models in the natural host.eLife · 2026Article
- High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells.Poultry science · 2026Article
- Biochemical components of chicken primordial germ cells are modified by cryopreservation: original analysis by Fourier Transform Infrared (FTIR) microspectroscopy.Poultry science · 2026Article
- NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis.Poultry science · 2026Article
- Efficient and Safe Knockout ofVeterinary sciences · 2026Article
- ATAC-seq of low-input and cryopreserved primordial germ cells reveals functional enhancers.Development (Cambridge, England) · 2026Article
- Heterochronic activation of TGF-β signaling drives the diversity of the avian sterna.Nature communications · 2026Article
- PGC-derived migrasomes couple PGC proliferation with migration.Nature communications · 2026Article
- Transcriptome Comparison Between the Cultured and In Vivo Chick Primordial Germ Cells by SMART-Seq-Based Single-Cell RNA Sequencing.Development, growth & differentiation · 2026Article
- Bleb-based extravasation uses conserved morphodynamics but divergent calcium control.Nature communications · 2026Article
- Advancing immunity and disease resistance in chickens through genome editing.Journal of animal science and biotechnology · 2026Review
55 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Precise self-renewal of the germ cell lineage is fundamental to fertility and reproductive success. The early precursors for the germ lineage, primordial germ cells (PGCs), survive and proliferate in several embryonic locations during their migration to the embryonic gonad. By elucidating the active signaling pathways in migratory PGCs in vivo, we were able to create culture conditions that recapitulate this embryonic germ cell environment. In defined medium conditions without feeder cells, the growth factors FGF2, insulin, and Activin A, signaling through their cognate-signaling pathways, were sufficient for self-renewal of germline-competent PGCs. Forced expression of constitutively active MEK1, AKT, and SMAD3 proteins could replace their respective upstream growth factors. Unexpectedly, we found that BMP4 could replace Activin A in non-clonal growth conditions. These defined medium conditions identify the key molecular pathways required for PGC self-renewal and will facilitate efforts in biobanking of chicken genetic resources and genome editing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.