Evidence map›Paper›PMID 26677255›Full record

ArticleJournal of clinical microbiology2016

Performance of Copan WASP for Routine Urine Microbiology.

Chantal Quiblier, Marion Jetter, Mark Rominski, Forouhar Mouttet, Erik C Böttger, Peter M Keller, Michael Hombach

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
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  5. Deep Learning-Based Rapid Identification ofRisk management and healthcare policy · 2026
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  11. Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept.International journal of molecular sciences · 2022
    Article
  12. Article
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  14. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chantal QuiblierUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland.
Marion JetterUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland.
Mark RominskiUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland.
Forouhar MouttetUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland.
Erik C BöttgerUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland.
Peter M KellerUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland.
Michael HombachUniversität Zürich, Institut für Medizinische Mikrobiologie, Zürich, Switzerland mhombach@imm.uzh.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study compared a manual workup of urine clinical samples with fully automated WASPLab processing. As a first step, two different inocula (1 and 10 μl) and different streaking patterns were compared using WASP and InoqulA BT instrumentation. Significantly more single colonies were produced with the10-μl inoculum than with the 1-μl inoculum, and automated streaking yielded significantly more single colonies than manual streaking on whole plates (P < 0.001). In a second step, 379 clinical urine samples were evaluated using WASP and the manual workup. Average numbers of detected morphologies, recovered species, and CFUs per milliliter of all 379 urine samples showed excellent agreement between WASPLab and the manual workup. The percentage of urine samples clinically categorized as positive or negative did not differ between the automated and manual workflow, but within the positive samples, automated processing by WASPLab resulted in the detection of more potential pathogens. In summary, the present study demonstrates that (i) the streaking pattern, i.e., primarily the number of zigzags/length of streaking lines, is critical for optimizing the number of single colonies yielded from primary cultures of urine samples; (ii) automated streaking by the WASP instrument is superior to manual streaking regarding the number of single colonies yielded (for 32.2% of the samples); and (iii) automated streaking leads to higher numbers of detected morphologies (for 47.5% of the samples), species (for 17.4% of the samples), and pathogens (for 3.4% of the samples). The results of this study point to an improved quality of microbiological analyses and laboratory reports when using automated sample processing by WASP and WASPLab.

Indexed as

Automation, LaboratoryHumansMicrobiological TechniquesSensitivity and SpecificityUrinary Tract InfectionsUrine

Identifiers

PMID26677255
PMCPMC4767997

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.