Evidence map›Paper›PMID 26676255›Full record

ArticlePlant physiology2016

Ester Cross-Link Profiling of the Cutin Polymer of Wild-Type and Cutin Synthase Tomato Mutants Highlights Different Mechanisms of Polymerization.

Glenn Philippe, Cédric Gaillard, Johann Petit, Nathalie Geneix, Michèle Dalgalarrondo, Cécile Bres, Jean-Philippe Mauxion, Rochus Franke, Christophe Rothan, Lukas Schreiber and 2 more

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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
6.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 66 citations in OpenAlex.

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  10. Suberin Biosynthesis, Assembly, and Regulation.Plants (Basel, Switzerland) · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Glenn PhilippeInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Cédric GaillardInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Johann PetitInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Nathalie GeneixInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Michèle DalgalarrondoInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Cécile BresInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Jean-Philippe MauxionInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Rochus FrankeInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Christophe RothanInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Lukas SchreiberInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).ORCID http://orcid.org/0000-0001-7003-9929
Didier MarionInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.).
Bénédicte BakanInstitut National de la Recherche Agronomique, Research Unit Biopolymers Interactions Assemblies, BP71627 44316, Nantes cedex 3, France (G.P., C.G., N.G., M.D., D.M., B.B.);Institut National de la Recherche Agronomique, University of Bordeaux, Unité Mixte de Recherche 1332 Fruit Biology and Pathology, 33140 Villenave d'Ornon, France (J.P., C.B., J.-P.M., C.R.); andInstitute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (R.F., L.S.) benedicte.bakan@nantes.inra.fr.
Université de Bordeaux · FRUniversity of Bonn · DEBiopolymères Interactions Assemblages · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cuticle function is closely related to the structure of the cutin polymer. However, the structure and formation of this hydrophobic polyester of glycerol and hydroxy/epoxy fatty acids has not been fully resolved. An apoplastic GDSL-lipase known as CUTIN SYNTHASE1 (CUS1) is required for cutin deposition in tomato (Solanum lycopersicum) fruit exocarp. In vitro, CUS1 catalyzes the self-transesterification of 2-monoacylglycerol of 9(10),16-dihydroxyhexadecanoic acid, the major tomato cutin monomer. This reaction releases glycerol and leads to the formation of oligomers with the secondary hydroxyl group remaining nonesterified. To check this mechanism in planta, a benzyl etherification of nonesterified hydroxyl groups of glycerol and hydroxy fatty acids was performed within cutin. Remarkably, in addition to a significant decrease in cutin deposition, mid-chain hydroxyl esterification of the dihydroxyhexadecanoic acid was affected in tomato RNA interference and ethyl methanesulfonate-cus1 mutants. Furthermore, in these mutants, the esterification of both sn-1,3 and sn-2 positions of glycerol was impacted, and their cutin contained a higher molar glycerol-to-dihydroxyhexadecanoic acid ratio. Therefore, in planta, CUS1 can catalyze the esterification of both primary and secondary alcohol groups of cutin monomers, and another enzymatic or nonenzymatic mechanism of polymerization may coexist with CUS1-catalyzed polymerization. This mechanism is poorly efficient with secondary alcohol groups and produces polyesters with lower molecular size. Confocal Raman imaging of benzyl etherified cutins showed that the polymerization is heterogenous at the fruit surface. Finally, by comparing tomato mutants either affected or not in cutin polymerization, we concluded that the level of cutin cross-linking had no significant impact on water permeance.

Indexed as

EsterificationEstersEthyl MethanesulfonateFatty AcidsFruitGlycerolLipaseMembrane LipidsMutationPlant ProteinsPolyestersPolymerizationPolymersSolanum lycopersicumcutinEstersEthyl MethanesulfonateFatty AcidsGlycerolLipaseMembrane LipidsPlant ProteinsPolyestersPolymers

Identifiers

PMID26676255
PMCPMC4734573
OpenAlexW2283959282

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.