Evidence map›Paper›PMID 26675981›Full record

ArticleBBA clinical2015

Preoperative protein profiles in cerebrospinal fluid in elderly hip fracture patients at risk for delirium: A proteomics and validation study.

Dunja Westhoff, Joost Witlox, Corneli van Aalst, Rikie M Scholtens, Sophia E de Rooij, Barbara C van Munster, Jos F M de Jonghe, Alexander P J Houdijk, Piet Eikelenboom, David J van Westerloo and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BBA clinical, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. Observational
  6. Article
  7. Review
  8. Article
  9. Article
  10. Development of a Dynamic Multi-Protein Signature of Postoperative Delirium.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 8 institutions in 2 countries.

Dunja WesthoffDepartment of Neurology, Academic Medical Center, PO box 22660, 1100 DD Amsterdam, The Netherlands.
Joost WitloxDepartment of Geriatrics, Medical Center Alkmaar, Wilhelminalaan 12, 1815 JD Alkmaar, The Netherlands.
Corneli van AalstDepartment of Respiratory Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
Rikie M ScholtensDepartment of Geriatrics, Academic Medical Center, PO box 22660, 1100 DD Amsterdam, The Netherlands.
Sophia E de RooijDepartment of Geriatrics, Academic Medical Center, PO box 22660, 1100 DD Amsterdam, The Netherlands ; Department of Geriatrics, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Barbara C van MunsterDepartment of Geriatrics, Academic Medical Center, PO box 22660, 1100 DD Amsterdam, The Netherlands ; Department of Geriatrics, Gelre hospitals, Albert Schweitzerlaan 31, 7334 DZ Apeldoorn, The Netherlands.
Jos F M de JongheDepartment of Geriatrics, Medical Center Alkmaar, Wilhelminalaan 12, 1815 JD Alkmaar, The Netherlands.
Alexander P J HoudijkDepartment of Surgery, Medical Center Alkmaar, Wilhelminalaan 12, 1815 JD Alkmaar, The Netherlands ; Trial Center Holland Health, Alkmaar, The Netherlands.
Piet EikelenboomGGZinGeest, Postbus 74077, 1070 BB Amsterdam, The Netherlands.
David J van WesterlooDepartment of Intensive Care Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
Diederik van de BeekDepartment of Neurology, Academic Medical Center, PO box 22660, 1100 DD Amsterdam, The Netherlands.
Willem A van GoolDepartment of Neurology, Academic Medical Center, PO box 22660, 1100 DD Amsterdam, The Netherlands.
Leo KoendermanDepartment of Respiratory Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
Academic Medical Center · NLMedisch Centrum Alkmaar · NLGelre Hospitals · NLGGZ inGeest · NLLeiden University Medical Center · NLUniversity Hospital Heidelberg · DEUniversity Medical Center Groningen · NLUniversity Medical Center Utrecht · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA neuroinflammatory response is suggested to play an important role in delirium, a common complication in older hospitalized patients. We examined whether hip fracture patients who develop postoperative delirium have a different proteome in cerebrospinal fluid (CSF) prior to surgery.

methodsPatients (≥ 75 years) were admitted for hip fracture surgery. CSF was collected during spinal anaesthesia; proteins were separated using gel electrophoresis and identified with mass spectrometry. We compared the proteome of patients with and without postoperative delirium. Findings were validated in an independent, comparable cohort using immuno-assays.

resultsIn the derivation cohort 53 patients were included, 35.8% developed postoperative delirium. We identified differences in levels of eight CSF proteins between patients with and without subsequent delirium: complement factor C3, contactin-1, fibulin-1 and I-beta-1,3-N-acetylglucosaminyltransferase were significantly lower in patients with postoperative delirium, while neural cell adhesion molecule-2, fibrinogen, zinc-α-2-glycoprotein and haptoglobin levels were significantly higher. In the validation cohort 21.2% of 52 patients developed postoperative delirium. Immuno-assays confirmed contactin-1 results although not statistically significant. Complement factor C3 was significantly higher in patients with postoperative delirium.

conclusionOur results show the complexity of pathophysiological mechanisms involved in delirium and emphasizes the need of independent validation of findings. GENERAL SIGNIFICANCE: This study highlights the challenges and inconsistent findings in studies of delirium, a serious complication in older patients. We analysed proteins in CSF, the most proximal fluid to the brain. All patients were free from delirium at the time of sampling.

Indexed as

2D-DIGE, Two-dimensional difference gel electrophoresisb3GNT3, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferaseC3, Third component of the complement systemCSFCSF, Cerebrospinal fluidDeliriumELISA, Enzyme linked immunosorbent assayIQR, Interquartile rangeLC-MS/MS, Liquid chromatography–mass spectrometryNeuroinflammationProteomicsSDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresisValidation

Identifiers

PMID26675981
PMCPMC4661734
OpenAlexW2201137641

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.