Evidence map›Paper›PMID 26675891›Full record

ArticleGigaScience2015

Spatially transformed fluorescence image data for ERK-MAPK and selected proteins within human epidermis.

Joseph Cursons, Catherine E Angel, Daniel G Hurley, Cristin G Print, P Rod Dunbar, Marc D Jacobs, Edmund J Crampin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in GigaScience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Joseph CursonsSystems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, Parkville, VIC Australia, 3010 ; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Australia, 3010.
Catherine E AngelMaurice Wilkins Centre, University of Auckland, Auckland, New Zealand ; School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Daniel G HurleySystems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, Parkville, VIC Australia, 3010.
Cristin G PrintMaurice Wilkins Centre, University of Auckland, Auckland, New Zealand ; School of Biological Sciences, University of Auckland, Auckland, New Zealand ; Bioinformatics Institute, University of Auckland, Auckland, New Zealand ; Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand.
P Rod DunbarMaurice Wilkins Centre, University of Auckland, Auckland, New Zealand ; School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Marc D JacobsDepartment of Biology, New Zealand International College, ACG New Zealand, Auckland, New Zealand.
Edmund J CrampinSystems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, Parkville, VIC Australia, 3010 ; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Australia, 3010 ; School of Mathematics and Statistics, University of Melbourne, Parkville, Australia, 3010 ; School of Medicine, University of Melbourne, Parkville, Australia, 3010.
Maurice Wilkins Centre · NZUniversity of Melbourne · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPhosphoprotein signalling pathways have been intensively studied in vitro, yet their role in regulating tissue homeostasis is not fully understood. In the skin, interfollicular keratinocytes differentiate over approximately 2 weeks as they traverse the epidermis. The extracellular signal-regulated kinase (ERK) branch of the mitogen-activated protein kinase (MAPK) pathway has been implicated in this process. Therefore, we examined ERK-MAPK activity within human epidermal keratinocytes in situ.

findingsWe used confocal microscopy and immunofluorescence labelling to measure the relative abundances of Raf-1, MEK1/2 and ERK1/2, and their phosphorylated (active) forms within three human skin samples. Additionally, we measured the abundance of selected proteins thought to modulate ERK-MAPK activity, including calmodulin, β1 integrin and stratifin (14-3-3σ); and of transcription factors known to act as effectors of ERK1/2, including the AP-1 components Jun-B, Fra2 and c-Fos. Imaging was performed with sufficient resolution to identify the plasma membrane, cytoplasm and nucleus as distinct domains within cells across the epidermis. The image field of view was also sufficiently large to capture the entire epidermis in cross-section, and thus the full range of keratinocyte differentiation in a single observation. Image processing methods were developed to quantify image data for mathematical and statistical analysis. Here, we provide raw image data and processed outputs.

conclusionsThese data indicate coordinated changes in ERK-MAPK signalling activity throughout the depth of the epidermis, with changes in relative phosphorylation-mediated signalling activity occurring along the gradient of cellular differentiation. We believe these data provide unique information about intracellular signalling as they are obtained from a homeostatic human tissue, and they might be useful for investigating intercellular heterogeneity.

Indexed as

MAP Kinase Signaling SystemEpidermal CellsEpidermisExtracellular Signal-Regulated MAP KinasesFluorescenceFos-Related Antigen-2HumansImage Processing, Computer-AssistedKeratinocytesMAP Kinase Kinase 1Mitogen-Activated Protein Kinase 3Proto-Oncogene Proteins c-fosTranscription Factor AP-1Transcription FactorsExtracellular Signal-Regulated MAP KinasesFos-Related Antigen-2JunB protein, humanMAP Kinase Kinase 1Mitogen-Activated Protein Kinase 3Proto-Oncogene Proteins c-fosTranscription Factor AP-1Transcription FactorsCalmodulinCellular heterogeneityConfocal microscopyHomeostatic tissueImmunofluorescenceInterfollicular keratinocytesMEK1/2Skin

Identifiers

PMID26675891
PMCPMC4678632
OpenAlexW2204014420

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.