Evidence map›Paper›PMID 26675708›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2016

Mechanical signals promote osteogenic fate through a primary cilia-mediated mechanism.

Julia C Chen, David A Hoey, Mardonn Chua, Raymond Bellon, Christopher R Jacobs

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
7.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 103 citations in OpenAlex.

  1. Ciliary-Mediated Mechanotransduction in Skeletal Development and Diseases.Results and problems in cell differentiation · 2026
    Review
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  3. Article
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  7. Article
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  9. Article
  10. Review
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  12. Cranium growth, patterning and homeostasis.Development (Cambridge, England) · 2022
    Article
  13. Role of Primary Cilia in Bone and Cartilage.Journal of dental research · 2022
    Article
  14. Review
  15. Biomechanical cues as master regulators of hematopoietic stem cell fate.Cellular and molecular life sciences : CMLS · 2021
    Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Julia C Chen*Department of Biomedical Engineering and Department of Chemical Engineering, Columbia University, New York, New York, USA; Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, and Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, Ireland; Department of Mechanical, Aeronautical, and Biomedical Engineering, Centre for Applied Biomedical Engineering Research, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland; and Department of Biotechnology, University of British Columbia, Vancouver, British Columbia, Canada.
David A Hoey*Department of Biomedical Engineering and Department of Chemical Engineering, Columbia University, New York, New York, USA; Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, and Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, Ireland; Department of Mechanical, Aeronautical, and Biomedical Engineering, Centre for Applied Biomedical Engineering Research, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland; and Department of Biotechnology, University of British Columbia, Vancouver, British Columbia, Canada david.hoey@ul.ie.
Mardonn Chua*Department of Biomedical Engineering and Department of Chemical Engineering, Columbia University, New York, New York, USA; Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, and Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, Ireland; Department of Mechanical, Aeronautical, and Biomedical Engineering, Centre for Applied Biomedical Engineering Research, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland; and Department of Biotechnology, University of British Columbia, Vancouver, British Columbia, Canada.
Raymond Bellon*Department of Biomedical Engineering and Department of Chemical Engineering, Columbia University, New York, New York, USA; Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, and Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, Ireland; Department of Mechanical, Aeronautical, and Biomedical Engineering, Centre for Applied Biomedical Engineering Research, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland; and Department of Biotechnology, University of British Columbia, Vancouver, British Columbia, Canada.
Christopher R Jacobs*Department of Biomedical Engineering and Department of Chemical Engineering, Columbia University, New York, New York, USA; Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, and Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, Ireland; Department of Mechanical, Aeronautical, and Biomedical Engineering, Centre for Applied Biomedical Engineering Research, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland; and Department of Biotechnology, University of British Columbia, Vancouver, British Columbia, Canada.
Columbia University · USUniversity of British Columbia · CAUniversity of Limerick · IE

Funding

Primary cilia as mechanotransducers in boneR01AR062177 · NIAMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GUO, X. EDWARD · 2012 to 2021
$3.9M
Multidisciplinary Engineering Training in Musculoskeletal ResearchT32AR059038 · NIAMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI ATESHIAN, GERARD A. · 2011 to 2015
$1.3M
Primary Cilia as Mechanotransducers in BoneR21AR054156 · NIAMS · PALO ALTO VETERANS INSTIT FOR RESEARCH · PI JACOBS, CHRISTOPHER RAE · 2007 to 2008
$328k
NIAMS NIH HHS AR054156NIAMS NIH HHS AR059038NIAMS NIH HHS AR062177NIAMS NIH HHS R01 AR062177NIAMS NIH HHS R21 AR054156NIAMS NIH HHS T32 AR059038
6 · The paper itself

Abstract

It has long been suspected, but never directly shown, that bone formed to accommodate an increase in mechanical loading is related to the creation of osteoblasts from skeletal stem cells. Indeed, biophysical stimuli potently regulate osteogenic lineage commitmentin vitro In this study, we transplanted bone marrow cells expressing green fluorescent protein, to enable lineage tracing, and subjected mice to a biophysical stimulus, to elicit a bone-forming response. We detected cells derived from transplanted progenitors embedded within the bone matrix near active bone-forming surfaces in response to loading, demonstrating for the first time, that mechanical signals enhance the homing and attachment of bone marrow cells to bone surfaces and the commitment to an osteogenic lineage of these cellsin vivo Furthermore, we used an inducible Cre/Lox recombination system to delete kinesin family member 3A (Kif3a), a gene that is essential for primary cilia formation, at will in transplanted cells and their progeny, regardless of which tissue may have incorporated them. Disruption of the mechanosensing organelle, the primary cilium in a progenitor population, significantly decreased the amount of bone formed in response to mechanical stimulation. The collective results of our study directly demonstrate that, in a novel experimental stem cell mechanobiology model, mechanical signals enhance osteogenic lineage commitmentin vivoand that the primary cilium contributes to this process.-Chen, J. C., Hoey, D. A., Chua, M., Bellon, R., Jacobs, C. R. Mechanical signals promote osteogenic fate through a primary cilia-mediated mechanism.

Indexed as

AnimalsBone Marrow CellsBone Marrow TransplantationCells, CulturedCiliaFlow CytometryGreen Fluorescent ProteinsImmunohistochemistryKinesinsMechanotransduction, CellularMesenchymal Stem CellsMice, Inbred C57BLMice, KnockoutMice, TransgenicOsteoblastsOsteogenesisGreen Fluorescent ProteinsKif3a protein, mouseKinesinsbonehomingmesenchymal stem cellprimary ciliumulna loading

Identifiers

PMID26675708
PMCPMC4799506
OpenAlexW2203792983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.