Evidence map›Paper›PMID 26675257›Full record

ArticleOncotarget2016

Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche.

Catherine A Sánchez, Eliana I Andahur, Rodrigo Valenzuela, Enrique A Castellón, Juan A Fullá, Christian G Ramos, Juan C Triviño

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
128citing papers in PubMed, 1 pooled it
9.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

128 citing papers in PubMed, 1 synthesis or guideline pooled it, 201 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review of the premetastatic niche in liver cancer bone metastases.International journal of clinical and experimental pathology · 2026
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  18. Theranostic signature of tumor-derived exosomes in cancer.Medical oncology (Northwood, London, England) · 2023
    Review
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  20. Harnessing cancer stem cell-derived exosomes to improve cancer therapy.Journal of experimental & clinical cancer research : CR · 2023
    Review

68 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Catherine A SánchezUrology Department, Las Condes Clinic, Santiago, Chile.
Eliana I AndahurUrology Department, Las Condes Clinic, Santiago, Chile.
Rodrigo ValenzuelaFaculty of Medicine, University of Chile, Santiago, Chile.
Enrique A CastellónFaculty of Medicine, University of Chile, Santiago, Chile.
Juan A FulláUrology Department, Las Condes Clinic, Santiago, Chile.
Christian G RamosUrology Department, Las Condes Clinic, Santiago, Chile.
Juan C TriviñoSistemas Genómicos, Valencia, España.
Clínica Las Condes · CLUniversity of Chile · CLSistemas Genómicos · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The different prostate cancer (PCa) cell populations (bulk and cancer stem cells, CSCs) release exosomes that contain miRNAs that could modify the local or premetastatic niche. The analysis of the differential expression of miRNAs in exosomes allows evaluating the differential biological effect of both populations on the niche, and the identification of potential biomarkers and therapeutic targets. Five PCa primary cell cultures were established to originate bulk and CSCs cultures. From them, exosomes were purified by precipitation for miRNAs extraction to perform a comparative profile of miRNAs by next generation sequencing in an Illumina platform. 1839 miRNAs were identified in the exosomes. Of these 990 were known miRNAs, from which only 19 were significantly differentially expressed: 6 were overexpressed in CSCs and 13 in bulk cells exosomes. miR-100-5p and miR-21-5p were the most abundant miRNAs. Bioinformatics analysis indicated that differentially expressed miRNAs are highly related with PCa carcinogenesis, fibroblast proliferation, differentiation and migration, and angiogenesis. Besides, miRNAs from bulk cells affects osteoblast differentiation. Later, their effect was evaluated in normal prostate fibroblasts (WPMY-1) where transfection with miR-100-5p, miR-21-5p and miR-139-5p increased the expression of metalloproteinases (MMPs) -2, -9 and -13 and RANKL and fibroblast migration. The higher effect was achieved with miR21 transfection. As conclusion, miRNAs have a differential pattern between PCa bulk and CSCs exosomes that act collaboratively in PCa progression and metastasis. The most abundant miRNAs in PCa exosomes are interesting potential biomarkers and therapeutic targets.

Indexed as

ApoptosisBiomarkers, TumorBlotting, WesternCell MovementCell ProliferationComputational BiologyDisease ProgressionExosomesFibroblastsGene Expression Regulation, NeoplasticHigh-Throughput Nucleotide SequencingHumansMaleMicroRNAsNeoplastic Stem CellsProstateBiomarkers, TumorMicroRNAsRNA, MessengerexosomesmiRNAsnext generation sequencingnicheprostate cancer

Identifiers

PMID26675257
PMCPMC4826185
OpenAlexW2264494318

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.