ArticleEpigenetics & chromatin2015
A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities.
Article in Epigenetics & chromatin, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Genetic and Epigenetic Mechanisms Underlying Reversible Adaptive Responses in Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- RiPP enzyme heterocomplex structure-guided discovery of a bacterial borosin α-RSC chemical biology · 2023Article
- Advances in direct detection of lysine methylation and acetylation by nuclear magnetic resonance usingMethods (San Diego, Calif.) · 2023Article
- A High-Throughput Continuous Spectroscopic Assay to Measure the Activity of Natural Product Methyltransferases.Chembiochem : a European journal of chemical biology · 2022Article
- Detection and Quantification of Histone Methyltransferase Activity In Vitro.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Probing multiple enzymatic methylation events in real time with NMR spectroscopy.Biophysical journal · 2021Article
- Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis.Nature communications · 2021Article
- Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification.Bioresources and bioprocessing · 2021Review
- Inhibition of Chikungunya virus by an adenosine analog targeting the SAM-dependent nsP1 methyltransferase.FEBS letters · 2020Article
- Lysine methylation signaling of non-histone proteins in the nucleus.Cellular and molecular life sciences : CMLS · 2019Review
- Forskolin attenuates doxorubicin-induced accumulation of asymmetric dimethylarginine and s-adenosylhomocysteineLeukemia research reports · 2018Article
- Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases.Frontiers in bioengineering and biotechnology · 2018Article
- A simplified characterization ofChemical science · 2017Article
- Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells.Scientific reports · 2016Article
- Article
- Detection of PRMT1 inhibitors with stopped flow fluorescence.Signal transduction and targeted therapyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMethyltransferases (MTs) catalyze the S-adenosylmethionine (SAM)-dependent methylation of a wide variety of protein and DNA substrates. Methylation of lysine, arginine or cytosine regulates a variety of biological processes including transcriptional activation and gene silencing. Despite extensive studies of the cellular roles of MTs, their quantitative kinetic characterization remains challenging. In the past decade, several assays have been developed to monitor methyl transfer activity utilizing different approaches including radiolabeling, antibodies or mass-spectrometry analysis. However, each approach suffers from different limitation and no easy continuous assay for detection of MT activity exists.
resultsWe have developed a continuous coupled assay for the general detection of MTs activity. In this assay, the formation of S-adenosylhomocysteine (SAH) product is coupled NAD(P)H oxidation through three enzyme reactions including glutamate dehydrogenase leading to absorbance changes at 340 nm. The utility and versatility of this assay is demonstrated for SET7/9 and SETD6 with peptides and full length protein substrates and for M.HaeIII with a DNA substrate.
conclusionsThis study shows a simple and robust assay for the continuous monitoring of MT enzymatic activity. This assay can be used for the determination of steady-state kinetic enzymatic parameters (e.g., k cat and K M) for a wide variety of MTs and can be easily adapted for high-throughput detection of MT activity for various applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.