Evidence map›Paper›PMID 26674844›Full record

ArticleEthnicity & disease2015

Effect of Interleukin-10 and Laminar Shear Stress on Endothelial Nitric Oxide Synthase and Nitric Oxide in African American Human Umbilical Vein Endothelial Cells.

Dianne M Babbitt, Ji-Seok Kim, Steven J Forrester, Michael D Brown, Joon-Young Park

Open access · bronzeAbstract read
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In one paragraph

Article in Ethnicity & disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Dianne M Babbitt1. Cedar Crest College, Allentown, Pennsylvania.
Ji-Seok Kim2. Department of Kinesiology, Temple University.
Steven J Forrester2. Department of Kinesiology, Temple University.
Michael D Brown3. Vascular Health Laboratory, Department of Kinesiology & Nutrition, University of Illinois at Chicago.
Joon-Young Park2. Department of Kinesiology, Temple University ; 4.Cardiovascular Research Center, Temple University School of Medicine.
Temple University · USCedar Crest College · USUniversity of Illinois Chicago · US

Funding

Genetics of In Vivo and In Vitro Endothelial Function in African AmericansR01HL085497 · NHLBI · TEMPLE UNIV OF THE COMMONWEALTH · PI BROWN, MICHAEL DAVID · 2007 to 2011
$3.3M
Functional implications of fluid shear stress-induced mitochondrial remodelingR01HL126952 · NHLBI · TEMPLE UNIV OF THE COMMONWEALTH · PI PARK, JOON YOUNG · 2015 to 2019
$2.1M
NHLBI NIH HHS R01 HL085497NHLBI NIH HHS R01 HL126952
6 · The paper itself

Abstract

backgroundAfrican Americans have a predisposition to heightened systemic inflammation and a high prevalence of hypertension.

objectiveThe purpose of this study was to evaluate the influence of interleukin-10 (IL-10) and laminar shear stress (LSS) on African American endothelial cells by measuring total endothelial nitric oxide synthase (eNOS) protein expression and its phosphorylated form (p-eNOS) at Serine 1177, and nitric oxide (NO) levels, in response to IL-10 incubation and high physiological levels of LSS, used as an in vitro mimetic for aerobic exercise training (AEXT).

designHuman umbilical vein endothelial cells (HUVEC) from an African American donor were cultured. The experimental conditions included Static, Static with IL-10 Incubation, LSS at 20 dynes/cm², and LSS at 20 dynes/cm² with IL-10 Incubation. Western blotting was used to measure eNOS and p-eNOS protein expression in the cells. A modified Griess assay was used to measure NO metabolites in the cell culture media.

resultsThere were significant increases in p-eNOS, eNOS, and NO in the LSS at 20 dynes/cm² and LSS at 20 dynes/cm² with IL-10 Incubation experimental conditions when compared to the Static experimental condition. There were no other statistically significant differences demonstrating that IL-10 did not have an additive effect on eNOS activity in our study.

conclusionThe significant increases in p-eNOS, eNOS, and NO as a result of LSS in African American HUVECs suggest that AEXT may be a viable, nonpharmacologic method to improve vascular inflammation status and vasodilation, and thereby contribute to hypertension reduction in the African American population.

Indexed as

Black or African AmericanStress, MechanicalCell Culture TechniquesHumansHuman Umbilical Vein Endothelial CellsInterleukin-10Nitric OxideNitric Oxide Synthase Type IIIShear StrengthIL10 protein, humanInterleukin-10Nitric OxideNitric Oxide Synthase Type IIINOS3 protein, humanAfrican AmericansEndothelial CellsEndothelial DysfunctionEndothelial Nitric Oxide SynthaseInflammationInterleukin-10Laminar Shear StressNitric Oxide

Identifiers

PMID26674844
PMCPMC4671427
OpenAlexW2201189359

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.