ArticleScientific reports2015
TALEN-mediated homologous recombination in Daphnia magna.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Effects of RNAi-by-feeding on DNA methyltransferases inEnvironmental epigenetics · 2026Article
- Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.Scientific reports · 2024Article
- Identification of gene isoforms and their switching events between male and female embryos of the parthenogenetic crustacean Daphnia magna.Scientific reports · 2024Article
- New advances in CRISPR/Cas-mediated precise gene-editing techniques.Disease models & mechanisms · 2023Review
- Regulation of Doublesex1 Expression for Environmental Sex Determination in the Cladoceran Crustacean Daphnia.Frontiers in cell and developmental biology · 2022Review
- Combining Old and New Tricks: The Study of Genes, Neurons, and Behavior in Crayfish.Frontiers in physiology · 2022Article
- Enhancing HR Frequency for Precise Genome Editing in Plants.Frontiers in plant science · 2022Article
- Production of genome-edited Daphnia for heavy metal detection by fluorescence.Scientific reports · 2020Article
- Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna.PloS one · 2020Article
- Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.PloS one · 2018Article
- Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.Scientific reports · 2017Article
- Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna.PLoS genetics · 2017Article
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.PloS one · 2017Article
- TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription Activator-Like Effector Nucleases (TALENs) offer versatile tools to engineer endogenous genomic loci in various organisms. We established a homologous recombination (HR)-based knock-in using TALEN in the crustacean Daphnia magna, a model for ecological and toxicological genomics. We constructed TALENs and designed the 67 bp donor insert targeting a point deletion in the eyeless mutant that shows eye deformities. Co-injection of the TALEN mRNA with donor DNA into eggs led to the precise integration of the donor insert in the germ line, which recovered eye deformities in offspring. The frequency of HR events in the germ line was 2% by using both plasmid and single strand oligo DNA with 1.5 kb and 80 nt homology to the target. Deficiency of ligase 4 involved in non-homologous end joining repair did not increase the HR efficiency. Our data represent efficient HR-based knock-in by TALENs in D. magna, which is a promising tool to understand Daphnia gene functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.