ArticleScientific reports2015
Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 68 citations in OpenAlex.
- Glucocorticoid treatment increases cholesterol availability during critical illness: effect on adrenal and muscle function.Critical care (London, England) · 2024Trial
- Potassium and the kidney.Nature reviews. Nephrology · 2026Review
- First European case of Geller syndrome complicated with hypokalemic nephropathy: A case report and literature review.Metabolism open · 2026Review
- Severe Diabetic Ketoacidosis With Refractory Hypernatremia due to Hypokalemia-Induced Arginine Vasopressin Resistance: A Case Report and Literature Review.Case reports in medicine · 2026Article
- Diagnosing apparent mineralocorticoid excess in a child with hypertension and nephrocalcinosis: from symptoms to genetics.CEN case reports · 2025Article
- A Contemporary Narrative Review of Sodium Homeostasis Mechanisms, Dysnatraemia, and the Clinical Relevance in Adult Critical Illness.Journal of clinical medicine · 2025Review
- Persistent Polyuria in Severe Hyponatremia Secondary to Beer Potomania and Hypokalemia: A Case Report.Cureus · 2025Article
- Type 1 Bartter syndrome presenting as primary diabetes insipidus: a rare Case Report with 8-year follow-up.Frontiers in genetics · 2025Article
- 'Aquaporin-omics': mechanisms of aquaporin-2 loss in polyuric disorders.The Journal of physiology · 2024Article
- Ectopic adrenocorticotropic hormone-secreting pheochromocytoma with severe metabolic disturbances: A case report.International journal of surgery case reports · 2024Article
- Hypokalemia Induced Partial Nephrogenic Diabetes Insipidus: A Case Report.JNMA; journal of the Nepal Medical Association · 2024Article
- Autophagy is involved in degradation of AQP1 in response to an acute decrement in tonicity.iScience · 2023Article
- Autophagy and regulation of aquaporins in the kidneys.Kidney research and clinical practice · 2023Article
- Preoperative management of patients with ectopic Cushing's syndrome caused by ACTH-secreting pheochromocytoma: a case series and review of the literature.Journal of endocrinological investigation · 2023Review
- Dissociation of sodium-chloride cotransporter expression and blood pressure during chronic high dietary potassium supplementation.JCI insight · 2023Article
- Mechanistic insights into the primary and secondary alterations of renal ion and water transport in the distal nephron.Journal of internal medicine · 2023Review
- Potassium and the kidney: a reciprocal relationship with clinical relevance.Pediatric nephrology (Berlin, Germany) · 2022Review
- Article
- Topiramate-induced severe electrolyte abnormalities and hypernatremia leading to central pontine myelinolysis.BMJ case reports · 2021Article
- Updates and Perspectives on Aquaporin-2 and Water Balance Disorders.International journal of molecular sciences · 2021Review
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Authors and funding
11 authors at 8 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypokalemia (low serum potassium level) is a common electrolyte imbalance that can cause a defect in urinary concentrating ability, i.e., nephrogenic diabetes insipidus (NDI), but the molecular mechanism is unknown. We employed proteomic analysis of inner medullary collecting ducts (IMCD) from rats fed with a potassium-free diet for 1 day. IMCD protein quantification was performed by mass spectrometry using a label-free methodology. A total of 131 proteins, including the water channel AQP2, exhibited significant changes in abundance, most of which were decreased. Bioinformatic analysis revealed that many of the down-regulated proteins were associated with the biological processes of generation of precursor metabolites and energy, actin cytoskeleton organization, and cell-cell adhesion. Targeted LC-MS/MS and immunoblotting studies further confirmed the down regulation of 18 selected proteins. Electron microscopy showed autophagosomes/autophagolysosomes in the IMCD cells of rats deprived of potassium for only 1 day. An increased number of autophagosomes was also confirmed by immunofluorescence, demonstrating co-localization of LC3 and Lamp1 with AQP2 and several other down-regulated proteins in IMCD cells. AQP2 was also detected in autophagosomes in IMCD cells of potassium-deprived rats by immunogold electron microscopy. Thus, enhanced autophagic degradation of proteins, most notably including AQP2, is an early event in hypokalemia-induced NDI.
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