ArticleScientific reports2015
Development of a real-time flexible multiphoton microendoscope for label-free imaging in a live animal.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed.
- Margin diagnosis for endoscopic submucosal dissection of early gastric cancer using multiphoton microscopy.Surgical endoscopy · 2020Trial
- Multiplexed video hyperspectral microendoscopy reveals tumor-immune dynamics in vivo.Research square · 2026Article
- Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy.Diagnostics (Basel, Switzerland) · 2026Review
- Hybrid endomicroscopic objective with monolithic multi-material achromatic triplet fabricated using two-photon lithography.Applied optics · 2026Article
- A handheld photoacoustic microscopic probe integrating a transparent ultrasound transducer and a fiber scanner.Nature communications · 2025Article
- The State of High-Resolution Imaging of the Human Inner Ear: A Look Into the Black Box.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Two-photon microscopy using picosecond pulses from four-wave mixing in a Yb-doped photonic crystal fiber.Biomedical optics express · 2025Article
- Video-rate two-photon microendoscopy using second harmonic resonance fiber scanning.Biomedical optics express · 2024Article
- Recent advances in light patterned optogenetic photostimulation in freely moving mice.Neurophotonics · 2024Article
- Advancing the path toNeurophotonics · 2024Article
- Perspectives on endoscopic functional photoacoustic microscopy.Applied physics letters · 2024Article
- Double-Clad Antiresonant Hollow-Core Fiber and Its Comparison with Other Fibers for Multiphoton Micro-Endoscopy.Sensors (Basel, Switzerland) · 2024Article
- Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration.Biophysical reviews · 2024Review
- Feasibility studies of multimodal nonlinear endoscopy using multicore fiber bundles for remote scanning from tissue sections to bulk organs.Scientific reports · 2023Article
- Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation.Journal of biomedical optics · 2023Article
- MEMS Enabled Miniature Two-Photon Microscopy for Biomedical Imaging.Micromachines · 2023Review
- Two-Photon Endoscopy: State of the Art and Perspectives.Molecular imaging and biology · 2023Review
- Label-Free Characterization and Quantification of Mucosal Inflammation in Common Murine Colitis Models With Multiphoton Imaging.Inflammatory bowel diseases · 2022Article
- Label-free highly multimodal nonlinear endoscope.Optics express · 2022Article
- Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning.Biomedical optics express · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a two-photon microendoscope capable of in vivo label-free deep-tissue high-resolution fast imaging through a very long optical fiber. First, an advanced light-pulse spectro-temporal shaping device optimally precompensates for linear and nonlinear distortions occurring during propagation within the endoscopic fiber. This enables the delivery of sub-40-fs duration infrared excitation pulses at the output of 5 meters of fiber. Second, the endoscopic fiber is a custom-made double-clad polarization-maintaining photonic crystal fiber specifically designed to optimize the imaging resolution and the intrinsic luminescence backward collection. Third, a miniaturized fiber-scanner of 2.2 mm outer diameter allows simultaneous second harmonic generation (SHG) and two-photon excited autofluorescence (TPEF) imaging at 8 frames per second. This microendoscope's transverse and axial resolutions amount respectively to 0.8 μm and 12 μm, with a field-of-view as large as 450 μm. This microendoscope's unprecedented capabilities are validated during label-free imaging, ex vivo on various fixed human tissue samples, and in vivo on an anesthetized mouse kidney demonstrating an imaging penetration depth greater than 300 μm below the surface of the organ. The results reported in this manuscript confirm that nonlinear microendoscopy can become a valuable clinical tool for real-time in situ assessment of pathological states.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.