ArticleNature communications2015
Ternary structure reveals mechanism of a membrane diacylglycerol kinase.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Nature Counts to Three: Universal Mg-Pinch Motif Polarizes the Cleaved Bond in NTP-Processing Enzymes.Journal of the American Chemical Society · 2026Article
- Modulators of Diacylglycerol Kinase Activity: A Review of Advances and Challenges.Medicinal research reviews · 2026Review
- Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX.IUCrJ · 2022Article
- Structure of membrane diacylglycerol kinase in lipid bilayers.Communications biology · 2021Article
- Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.Structure (London, England : 1993) · 2020Article
- Folding and Misfolding of Human Membrane Proteins in Health and Disease: From Single Molecules to Cellular Proteostasis.Chemical reviews · 2019Article
- Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.Chemical reviews · 2019Review
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
- Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR.Scientific reports · 2019Article
- Diacylglycerol kinases: Relationship to other lipid kinases.Advances in biological regulation · 2019Review
- Membrane properties that shape the evolution of membrane enzymes.Current opinion in structural biology · 2018Review
- Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis.Nature communications · 2018Article
- Comparison of NMR and crystal structures of membrane proteins and computational refinement to improve model quality.Proteins · 2018Article
- Structural basis for catalysis at the membrane-water interface.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- A Bright Future for Serial Femtosecond Crystallography with XFELs.Trends in biochemical sciences · 2017Review
- Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.Nature communications · 2016Article
- Serial femtosecond crystallography: A revolution in structural biology.Archives of biochemistry and biophysics · 2016Review
- In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.Acta crystallographica. Section D, Structural biology · 2016Article
Corrections and comments
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Authors and funding
30 authors at 10 institutions in 4 countries.
Funding
Abstract
Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available protein must be used economically to craft three catalytic and substrate-binding sites centred about the membrane/cytosol interface. How nature has accomplished this extraordinary feat is revealed here in a crystal structure of the kinase captured as a ternary complex with bound lipid substrate and an ATP analogue. Residues, identified as essential for activity by mutagenesis, decorate the active site and are rationalized by the ternary structure. The γ-phosphate of the ATP analogue is positioned for direct transfer to the primary hydroxyl of the lipid whose acyl chain is in the membrane. A catalytic mechanism for this unique enzyme is proposed. The active site architecture shows clear evidence of having arisen by convergent evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.