ArticleNucleic acids research2016
DNA recognition for virus assembly through multiple sequence-independent interactions with a helix-turn-helix motif.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Isolation and molecular characterization of bacteriophages isolated from lake water and their application in foods against Bacillus cereus.BMC research notes · 2025Article
- Genomic and Functional Characterization of Novel Phages Targeting Multidrug-ResistantInternational journal of molecular sciences · 2025Article
- Structural basis for DNA recognition by a viral genome-packaging machine.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterization and genomic analysis of a novel lytic phage vB_PstM_ZRG1 infecting Stutzerimonas stutzeri, representing a new viral genus, Elithevirus.Virus research · 2023Article
- Viral Small Terminase: A Divergent Structural Framework for a Conserved Biological Function.Viruses · 2022Review
- Isolation, characterization, and application of a novel polyvalent lytic phage STWB21 against typhoidal and nontyphoidalFrontiers in microbiology · 2022Article
- The Fork Protection Complex: A Regulatory Hub at the Head of the Replisome.Sub-cellular biochemistry · 2022Article
- Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Biophysical analysis of Pseudomonas-phage PaP3 small terminase suggests a mechanism for sequence-specific DNA-binding by lateral interdigitation.Nucleic acids research · 2020Article
- Crystal structure and interactions of the Tof1-Csm3 (Timeless-Tipin) fork protection complex.Nucleic acids research · 2020Article
- Isolation, Characterization and Genomic Analysis of a Novel Bacteriophage VB_EcoS-Golestan Infecting Multidrug-Resistant Escherichia coli Isolated from Urinary Tract Infection.Scientific reports · 2020Article
- A thermophilic phage uses a small terminase protein with a fixed helix-turn-helix geometry.The Journal of biological chemistry · 2020Article
- Identification of the First Gene Transfer Agent (GTA) Small Terminase inJournal of virology · 2019Article
- Article
- Viral genome packaging terminase cleaves DNA using the canonical RuvC-like two-metal catalysis mechanism.Nucleic acids research · 2017Article
- A β-Mannanase with a Lysozyme-like Fold and a Novel Molecular Catalytic Mechanism.ACS central science · 2016Article
- Identification of DNA-binding proteins using multi-features fusion and binary firefly optimization algorithm.BMC bioinformatics · 2016Article
- DNA Topology and the Initiation of Virus DNA Packaging.PloS one · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
The helix-turn-helix (HTH) motif features frequently in protein DNA-binding assemblies. Viral pac site-targeting small terminase proteins possess an unusual architecture in which the HTH motifs are displayed in a ring, distinct from the classical HTH dimer. Here we investigate how such a circular array of HTH motifs enables specific recognition of the viral genome for initiation of DNA packaging during virus assembly. We found, by surface plasmon resonance and analytical ultracentrifugation, that individual HTH motifs of the Bacillus phage SF6 small terminase bind the packaging regions of SF6 and related SPP1 genome weakly, with little local sequence specificity. Nuclear magnetic resonance chemical shift perturbation studies with an arbitrary single-site substrate suggest that the HTH motif contacts DNA similarly to how certain HTH proteins contact DNA non-specifically. Our observations support a model where specificity is generated through conformational selection of an intrinsically bent DNA segment by a ring of HTHs which bind weakly but cooperatively. Such a system would enable viral gene regulation and control of the viral life cycle, with a minimal genome, conferring a major evolutionary advantage for SPP1-like viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.