ArticleNucleic acids research2016
Functional dissection of a plant Argonaute.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 63 citations in OpenAlex.
- Disabling posttranscriptional regulation of AGO2 results in enhanced viral resistance.The New phytologist · 2026Article
- Epigenetic control of seed development and dormancy in cereals.Plant signaling & behavior · 2025Review
- A new level of RNA-based plant protection: dsRNAs designed from functionally characterized siRNAs highly effective against Cucumber mosaic virus.Nucleic acids research · 2025Article
- Function and regulation of plant ARGONAUTE proteins in response to environmental challenges: a review.PeerJ · 2024Review
- Hierarchical contribution of Argonaute proteins to antiviral protection.Journal of experimental botany · 2023Article
- RNA and Protein Determinants Mediate Differential Binding of miRNAs by a Viral Suppressor of RNA Silencing Thus Modulating Antiviral Immune Responses in Plants.International journal of molecular sciences · 2022Article
- NbNAC42 and NbZFP3 Transcription Factors Regulate the Virus InducibleFrontiers in plant science · 2022Article
- Defense-Related Gene Expression Following an Orthotospovirus Infection Is Influenced by Host Resistance inViruses · 2021Article
- Argonaute 5 family proteins play crucial roles in the defence against Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis aphrodite subsp. formosana.Molecular plant pathology · 2021Article
- Geminiviral Triggers and Suppressors of Plant Antiviral Immunity.Microorganisms · 2021Review
- Genome-wide identification of Argonautes in Solanaceae with emphasis on potato.Scientific reports · 2020Article
- The potyviral silencing suppressor HCPro recruits and employs host ARGONAUTE1 in pro-viral functions.PLoS pathogens · 2020Article
- Genome-Wide Identification of RNA Silencing-Related Genes and Their Expressional Analysis in Response to Heat Stress in Barley (Biomolecules · 2020Article
- Double-Stranded-RNA-Binding Protein 2 Participates in Antiviral Defense.Journal of virology · 2020Article
- Interspecies Communication in Holobionts by Non-Coding RNA Exchange.International journal of molecular sciences · 2020Review
- Integrated miRNAome and Transcriptome Analysis Reveals Argonaute 2-Mediated Defense Responses Against the Devastating PhytopathogenFrontiers in plant science · 2020Article
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Highly efficacious antiviral protection of plants by small interfering RNAs identified in vitro.Nucleic acids research · 2019Article
- Extensive profiling inRNA (New York, N.Y.) · 2019Article
- Chemical enhancers of posttranscriptional gene silencing inRNA (New York, N.Y.) · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA guided ribonuclease complexes play central role in RNA interference. Members of the evolutionarily conserved Argonaute protein family form the catalytic cores of these complexes. Unlike a number of other plant Argonautes, the role of AGO2 has been obscure until recently. Newer data, however, have indicated its involvement in various biotic and abiotic stress responses. Despite its suggested importance, there is no detailed characterization of this protein to date. Here we report cloning and molecular characterization of the AGO2 protein of the virological model plant Nicotiana benthamiana. We show that AGO2 can directly repress translation via various miRNA target site constellations (ORF, 3' UTR). Interestingly, although AGO2 seems to be able to silence gene expression in a slicing independent fashion, its catalytic activity is still a prerequisite for efficient translational repression. Additionally, mismatches between the 3' end of the miRNA guide strand and the 5' end of the target site enhance gene silencing by AGO2. Several functionally important amino acid residues of AGO2 have been identified that affect its small RNA loading, cleavage activity, translational repression potential and antiviral activity. The data presented here help us to understand how AGO2 aids plants to deal with stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.