ArticleNucleic acids research2016
Synthetic dual-input mammalian genetic circuits enable tunable and stringent transcription control by chemical and light.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction.Nature communications · 2023Article
- A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila.ACS synthetic biology · 2023Article
- Synthetic Gene Circuit-Based Assay with Multilevel Switch Enables Background-Free and Absolute Quantification of Circulating Tumor DNA.Research (Washington, D.C.) · 2023Article
- Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins.Nature biotechnology · 2022Article
- Dual Systems for Enhancing Control of Protein Activity through Induced Dimerization Approaches.Advanced biology · 2021Review
- A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice.Nature communications · 2021Article
- Small-molecule inducible transcriptional control in mammalian cells.Critical reviews in biotechnology · 2020Review
- A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells.Nucleic acids research · 2020Article
- Mapping molecular pathways for embryonic Sertoli cells derivation based on differentiation model of mouse embryonic stem cells.Stem cell research & therapy · 2020Article
- Synthetic immunity by remote control.Theranostics · 2020Review
- Phenotype and target-based chemical biology investigations in cancers.National science review · 2019Article
- Noise-reducing optogenetic negative-feedback gene circuits in human cells.Nucleic acids research · 2019Article
- Development of bicistronic expression system for the enhanced and reliable production of recombinant proteins in Leuconostoc citreum.Scientific reports · 2018Article
- Design and implementation of a synthetic pre-miR switch for controlling miRNA biogenesis in mammals.Nucleic acids research · 2017Article
- Synthetic regulatory RNAs selectively suppress the progression of bladder cancer.Journal of experimental & clinical cancer research : CR · 2017Article
- Mammalian synthetic biology in the age of genome editing and personalized medicine.Current opinion in chemical biology · 2017Review
- Conditional control of suicide gene expression in tumor cells with theophylline-responsive ribozyme.Gene therapy · 2017Article
- Synthetic circuits that process multiple light and chemical signal inputs.BMC systems biology · 2017Article
- Quantitative Systems Biology to decipher design principles of a dynamic cell cycle network: the "Maximum Allowable mammalian Trade-Off-Weight" (MAmTOW).NPJ systems biology and applications · 2017Review
- Small-molecule control of protein function through Staudinger reduction.Nature chemistry · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Programmable transcription factors can enable precise control of gene expression triggered by a chemical inducer or light. To obtain versatile transgene system with combined benefits of a chemical inducer and light inducer, we created various chimeric promoters through the assembly of different copies of the tet operator and Gal4 operator module, which simultaneously responded to a tetracycline-responsive transcription factor and a light-switchable transactivator. The activities of these chimeric promoters can be regulated by tetracycline and blue light synergistically or antagonistically. Further studies of the antagonistic genetic circuit exhibited high spatiotemporal resolution and extremely low leaky expression, which therefore could be used to spatially and stringently control the expression of highly toxic protein Diphtheria toxin A for light regulated gene therapy. When transferring plasmids engineered for the gene switch-driven expression of a firefly luciferase (Fluc) into mice, the Fluc expression levels of the treated animals directly correlated with the tetracycline and light input program. We suggest that dual-input genetic circuits using TET and light that serve as triggers to achieve expression profiles may enable the design of robust therapeutic gene circuits for gene- and cell-based therapies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.