ArticleNucleic acids research2016
A transcription-independent epigenetic mechanism is associated with antigenic switching in Trypanosoma brucei.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- A non-coding role for trypanosome VSG transcripts in allelic exclusion.Nucleic acids research · 2025Article
- Article
- Article
- TelAP2 links TelAP1 to the telomere complex in Trypanosoma brucei.Scientific reports · 2024Article
- Adipocyte lipolysis protects mice against Trypanosoma brucei infection.Nature microbiology · 2023Article
- Advances in Protozoan Epigenetic Targets and Their Inhibitors for the Development of New Potential Drugs.Pharmaceuticals (Basel, Switzerland) · 2023Review
- A long noncoding RNA promotes parasite differentiation in African trypanosomes.Science advances · 2022Article
- VEX1 Influences mVSG Expression During the Transition to Mammalian Infectivity inFrontiers in cell and developmental biology · 2022Article
- DNA double strand break position leads to distinct gene expression changes and regulates VSG switching pathway choice.PLoS pathogens · 2021Article
- The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands.PLoS pathogens · 2021Article
- May the Odds Be Ever in Your Favor: Non-deterministic Mechanisms Diversifying Cell Surface Molecule Expression.Frontiers in cell and developmental biology · 2021Review
- Trypanosoma brucei ATR Links DNA Damage Signaling during Antigenic Variation with Regulation of RNA Polymerase I-Transcribed Surface Antigens.Cell reports · 2020Article
- African trypanosomes expressing multiple VSGs are rapidly eliminated by the host immune system.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Dynamic colocalization of 2 simultaneously activeProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex.Nature communications · 2019Article
- Overexpression of Trypanosoma cruzi High Mobility Group B protein (TcHMGB) alters the nuclear structure, impairs cytokinesis and reduces the parasite infectivity.Scientific reports · 2019Article
- Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion.PLoS genetics · 2018Article
- TelAP1 links telomere complexes with developmental expression site silencing in African trypanosomes.Nucleic acids research · 2018Article
- How to create coats for all seasons: elucidating antigenic variation in African trypanosomes.Emerging topics in life sciences · 2017Article
- Serological tests for gambiense human African trypanosomiasis detect antibodies in cattle.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antigenic variation in Trypanosoma brucei relies on periodic switching of variant surface glycoproteins (VSGs), which are transcribed monoallelically by RNA polymerase I from one of about 15 bloodstream expression sites (BES). Chromatin of the actively transcribed BES is depleted of nucleosomes, but it is unclear if this open conformation is a mere consequence of a high rate of transcription, or whether it is maintained by a transcription-independent mechanism. Using an inducible BES-silencing reporter strain, we observed that chromatin of the active BES remains open for at least 24 hours after blocking transcription. This conformation is independent of the cell-cycle stage, but dependent upon TDP1, a high mobility group box protein. For two days after BES silencing, we detected a transient and reversible derepression of several silent BESs within the population, suggesting that cells probe other BESs before commitment to one, which is complete by 48 hours. FACS sorting and subsequent subcloning confirmed that probing cells are switching intermediates capable of returning to the original BES, switch to the probed BES or to a different BES. We propose that regulation of BES chromatin structure is an epigenetic mechanism important for successful antigenic switching.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.