ArticleCell reports2015
Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux.
Article in Cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Calcium Signaling in Macrophages During a Wound Response In Vivo.International journal of molecular sciences · 2026Article
- Hair Calcium Levels in Relation to Coronary Artery Disease Severity and Systemic Inflammation Markers: A Pilot Study.Journal of clinical medicine · 2025Article
- Mitochondrial metabolism is rapidly re-activated in mature neutrophils to support stimulation-induced response.bioRxiv : the preprint server for biology · 2025Article
- Mitochondrial metabolism is rapidly re-activated in mature neutrophils to support stimulation-induced response.Frontiers in immunology · 2025Article
- Review
- Zebrafish: an underutilized tool for discovery in host-microbe interactions.Trends in immunology · 2022Review
- Anomalous diffusion and asymmetric tempering memory in neutrophil chemotaxis.PLoS computational biology · 2022Article
- Real-time imaging of inflammation and its resolution: It's apparent because it's transparent.Immunological reviews · 2022Review
- Single-molecule studies reveal regulatory interactions between master kinases PDK1, AKT1, and PKC.Biophysical journal · 2021Article
- Article
- TRPM2 ion channels steer neutrophils towards a source of hydrogen peroxide.Scientific reports · 2021Article
- Neutrophil Swarming in Damaged Tissue Is Orchestrated by Connexins and Cooperative Calcium Alarm Signals.Current biology : CB · 2020Article
- The cell biology of inflammation: From common traits to remarkable immunological adaptations.The Journal of cell biology · 2020Review
- Illuminating Macrophage Contributions to Host-Pathogen InteractionsInfection and immunity · 2020Review
- Protective effect of cabergoline on mitochondrial oxidative stress-induced apoptosis is mediated by modulations of TRPM2 in neutrophils of patients with endometriosis.Journal of bioenergetics and biomembranes · 2020Article
- Role of the Intracellular Sodium Homeostasis in Chemotaxis of Activated Murine Neutrophils.Frontiers in immunology · 2020Article
- Mechanical features of endothelium regulate cell adhesive molecule-induced calcium response in neutrophils.APL bioengineering · 2019Article
- Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization.PLoS pathogens · 2019Article
- Calcium Signaling in Vertebrate Development and Its Role in Disease.International journal of molecular sciences · 2018Review
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
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7 authors at 2 institutions in 1 country.
Funding
Abstract
Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafish, we observe characteristic patterns of calcium flux triggered by distinct events, including phagocytosis of pathogenic bacteria and migration of neutrophils toward inflammatory stimuli. In contrast to findings from ex vivo studies, we observe enriched calcium influx at the leading edge of migrating neutrophils. To directly manipulate calcium dynamics in vivo, we have developed transgenic lines with cell-specific expression of the mammalian TRPV1 channel, enabling ligand-gated, reversible, and spatiotemporal control of calcium influx. We find that controlled calcium influx can function to help define the neutrophil's leading edge. Cell-specific TRPV1 expression may have broad utility for precise control of calcium dynamics in other immune cell types and organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.