ArticleMelanoma research2016
AHNAK is downregulated in melanoma, predicts poor outcome, and may be required for the expression of functional cadherin-1.
Article in Melanoma research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- Meta-cancer phosphoproteomic analysis unveils association of Tau phosphosites with DNA damage response.Frontiers in systems biology · 2026Article
- Downregulation of AHNAK2 inhibits cell cycle of lung adenocarcinoma cells by interacting with RUVBL1.Thoracic cancer · 2023Article
- AHNAKs roles in physiology and malignant tumors.Frontiers in oncology · 2023Review
- AHNAK Contributes to Hepatocellular Carcinoma Growth by Interacting with IGF-1R.Molecules (Basel, Switzerland) · 2022Article
- Synchronous polyostotic solid variant of aneurysmal bone cyst involving thoracic vertebrae and harboring a novel AHNAK::USP6 gene fusion. A case report and review.Virchows Archiv : an international journal of pathology · 2022Article
- Characterization of novel CTNNB1 mutation in Craniopharyngioma by whole-genome sequencing.Molecular cancer · 2021Article
- Review
- SMARCA4 promotes benign skin malignant transformation into melanoma through Adherens junction signal transduction.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021Article
- Article
- Non‑invasive proteome‑wide quantification of skin barrier‑related proteins using label‑free LC‑MS/MS analysis.Molecular medicine reports · 2020Article
- AHNAK2 Is Associated with Poor Prognosis and Cell Migration in Lung Adenocarcinoma.BioMed research international · 2020Article
- Identification of 9-Core Immune-Related Genes in Bladder Urothelial Carcinoma Prognosis.Frontiers in oncology · 2020Article
- Caveolin-1 in skin aging - From innocent bystander to major contributor.Ageing research reviews · 2019Review
- Paucimannosidic glycoepitopes inhibit tumorigenic processes in glioblastoma multiforme.Oncotarget · 2019Article
- Overexpression of RNF38 facilitates TGF-β signaling by Ubiquitinating and degrading AHNAK in hepatocellular carcinoma.Journal of experimental & clinical cancer research : CR · 2019Article
- Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition.Journal of Cancer · 2019Article
- Article
- Distinctive pattern of AHNAK methylation level in peripheral blood mononuclear cells and the association with HBV-related liver diseases.Cancer medicine · 2018Article
- Quantitative Proteomic Analysis Identifies AHNAK (Neuroblast Differentiation-associated Protein AHNAK) as a Novel Candidate Biomarker for Bladder Urothelial Carcinoma Diagnosis by Liquid-based Cytology.Molecular & cellular proteomics : MCP · 2018Article
- A risk score staging system based on the expression of seven genes predicts the outcome of bladder cancer.Oncology letters · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to further our understanding of the transformation process by identifying differentially expressed proteins in melanocytes compared with melanoma cell lines. Tandem mass spectrometry incorporating iTRAQ reagents was used as a screen to identify and comparatively quantify the expression of proteins in membrane-enriched samples isolated from primary human melanocytes or three melanoma cells lines. Real-time PCR was used to validate significant hits. Immunohistochemistry was used to validate the expression of proteins of interest in melanocytes in human skin and in melanoma-infiltrated lymph nodes. Publically available databases were examined to assess mRNA expression and correlation to patient outcome in a larger cohort of samples. Finally, preliminary functional studies were carried out using siRNAs to reduce the expression of a protein of interest in primary melanocytes and in a keratinocyte cell line. Two proteins, AHNAK and ANXA2, were significantly downregulated in the melanoma cell lines compared with melanocytes. Downregulation was confirmed in tumor cells in a subset of human melanoma-infiltrated human lymph nodes compared with melanocytes in human skin. Examination of Gene Expression Omnibus database data sets suggests that downregulation of AHNAK mRNA and mutation of the AHNAK gene are common in metastatic melanoma and correlates to a poor outcome. Knockdown of AHNAK in primary melanocytes and in a keratinocyte cell line led to a reduction in detectable cadherin-1. This is the first report that we are aware of which correlates a loss of AHNAK with melanoma and poor patient outcome. We hypothesize that AHNAK is required for the expression of functional cadherin-1.
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