ArticleThe Plant cell2015
KONJAC1 and 2 Are Key Factors for GDP-Mannose Generation and Affect l-Ascorbic Acid and Glucomannan Biosynthesis in Arabidopsis.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.Plant physiology · 2026Article
- Unraveling mannan biosynthesis in poplar and spruce: functional characterization of Cellulose Synthase-Like family A (CSLA) genes.The New phytologist · 2026Article
- Integrated physiological, biochemical and transcriptomic analyses revealed key genes regulating ascorbic acid biosynthesis during sweet potato development.Food chemistry. Molecular sciences · 2025Article
- The rice BRITTLE CULM 4 gene encodes a membrane protein affecting cellulose synthesis in the secondary cell wall.Plant & cell physiology · 2025Article
- A GDP-mannose-1-phosphate guanylyltransferase as a potential HIGS target against Sclerotinia sclerotiorum.PLoS pathogens · 2025Article
- Soluble sugars make a greater contribution than cell wall components to the variability of freezing tolerance in wheat cultivars.Plant biotechnology (Tokyo, Japan) · 2024Article
- Expression of laccase and ascorbate oxidase affects lignin composition in Arabidopsis thaliana stems.Journal of plant research · 2024Article
- Genomes of diverse Actinidia species provide insights into cis-regulatory motifs and genes associated with critical traits.BMC biology · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Ascorbic acid-mediated reactive oxygen species homeostasis modulates the switch from tapetal cell division to cell differentiation in Arabidopsis.The Plant cell · 2023Article
- Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN inFrontiers in plant science · 2023Article
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Glucomannan inGenes · 2022Review
- F-box protein MdAMR1L1 regulates ascorbate biosynthesis in apple by modulating GDP-mannose pyrophosphorylase.Plant physiology · 2022Article
- Crystal Structures ofFrontiers in plant science · 2022Article
- The perils of planning strategies to increase vitamin C content in plants: Beyond the hype.Frontiers in plant science · 2022Review
- Elevating Ascorbate in Arabidopsis Stimulates the Production of Abscisic Acid, Phaseic Acid, and to a Lesser Extent Auxin (IAA) and Jasmonates, Resulting in Increased Expression ofInternational journal of molecular sciences · 2021Article
- The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.Plant physiology · 2021Article
- A CCAAT-binding factor, SlNFYA10, negatively regulates ascorbate accumulation by modulating the D-mannose/L-galactose pathway in tomato.Horticulture research · 2020Article
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
Humans are unable to synthesize l-ascorbic acid (AsA), yet it is required as a cofactor in many critical biochemical reactions. The majority of human dietary AsA is obtained from plants. In Arabidopsis thaliana, a GDP-mannose pyrophosphorylase (GMPP), VITAMIN C DEFECTIVE1 (VTC1), catalyzes a rate-limiting step in AsA synthesis: the formation of GDP-Man. In this study, we identified two nucleotide sugar pyrophosphorylase-like proteins, KONJAC1 (KJC1) and KJC2, which stimulate the activity of VTC1. The kjc1kjc2 double mutant exhibited severe dwarfism, indicating that KJC proteins are important for growth and development. The kjc1 mutation reduced GMPP activity to 10% of wild-type levels, leading to a 60% reduction in AsA levels. On the contrary, overexpression of KJC1 significantly increased GMPP activity. The kjc1 and kjc1kjc2 mutants also exhibited significantly reduced levels of glucomannan, which is also synthesized from GDP-Man. Recombinant KJC1 and KJC2 enhanced the GMPP activity of recombinant VTC1 in vitro, while KJCs did not show GMPP activity. Yeast two-hybrid assays suggested that the stimulation of GMPP activity occurs via interaction of KJCs with VTC1. These results suggest that KJCs are key factors for the generation of GDP-Man and affect AsA level and glucomannan accumulation through the stimulation of VTC1 GMPP activity.
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