Evidence map›Paper›PMID 26671766›Full record

ArticleNeurochemical research2016

Changes in Lipid Composition During Manganese-Induced Apoptosis in PC12 Cells.

P A Corsetto, G Ferrara, S Buratta, L Urbanelli, G Montorfano, A Gambelunghe, E Chiaradia, A Magini, P Roderi, I Colombo and 2 more

Abstract read
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Article in Neurochemical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

P A CorsettoLaboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
G FerraraLaboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
S BurattaLaboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
L UrbanelliLaboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
G MontorfanoLaboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
A GambelungheDepartment of Medicine, School of Medicine, University of Perugia, Perugia, Italy.
E ChiaradiaDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
A MaginiLaboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy.
P RoderiLaboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
I ColomboLaboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy.
A M RizzoLaboratory of Membrane Biochemistry and Applied Nutrition, Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134, Milan, Italy. angelamaria.rizzo@unimi.it.
C EmilianiLaboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123, Perugia, Italy. carla.emiliani@unipg.it.
University of Perugia · ITUniversity of Milan · ITInstitute of Nanostructured Materials · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipid composition of membranes is fundamental to modulate signaling pathways relying on lipid metabolites and/or membrane proteins, thus resulting in the regulation of important cell processes such as apoptosis. In this case, membrane remodeling is an early event important for the activation of signaling leading to cell death and removal of apoptotic cells. In the present study, we analyzed phospholipid, cholesterol and fatty acid content during apoptosis induced by manganese in PC12 cells. Lipid analysis of whole cells and detergent-resistant membranes was carried out by HPLC/GC. Results showed that apoptosis is associated with changes in lipid composition detectable in whole cell extracts, namely cholesterol, phosphatidylserine and phosphatidylethanolamine decreases. Noteworthy, phosphatidylserine level reduction was detectable before to the detection of apoptosis, in correlation with our previous study carried out by radioactive labelling. By contrast, phosphatidylserine and phosphatidylethanolamine changes were not detected in detergent resistant membranes, which instead showed an altered composition in phosphatidylinositol, phosphatidylcholine and sphingomyelin in apoptotic cells.

Indexed as

Lipid MetabolismAnimalsApoptosisChloridesManganeseManganese CompoundsPC12 CellsPhospholipidsRatsSignal TransductionChloridesManganesemanganese chlorideManganese CompoundsPhospholipidsApoptosisDetergent-resistant membranesManganesePC12 cellsPhosphatidylserinePhospholipid composition

Identifiers

PMID26671766
OpenAlexW2252971779

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.