ArticleScientific reports2015
Proteome Analysis of Ground State Pluripotency.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Exploration of Serum Proteomic Profiling and Diagnostic Model That Differentiate Crohn's Disease and Intestinal Tuberculosis.PloS one · 2016Trial
- Ccar1 prevents β-catenin nuclear translocation to sustain ground-state pluripotency in mouse ESCs under R2i.Biochemistry and biophysics reports · 2025Article
- Claudin-6 mediates an embryonic stem cell-driven antitumor response against breast cancer.iScience · 2025Article
- RNA-Mediated Regulation of Glycolysis in Embryonic Stem Cell Pluripotency and Differentiation.Current stem cell reports · 2024Article
- Characterization of a Distinct State in the Continuum of Pluripotency Facilitated by Inhibition of PKCζ in Mouse Embryonic Stem Cells.Stem cell reviews and reports · 2023Article
- PeptideWitch-A Software Package to Produce High-Stringency Proteomics Data Visualizations from Label-Free Shotgun Proteomics Data.Proteomes · 2020Article
- Parallel bimodal single-cell sequencing of transcriptome and chromatin accessibility.Genome research · 2020Article
- Analysis of Hub Genes Involved in Distinction Between Aged and Fetal Bone Marrow Mesenchymal Stem Cells by Robust Rank Aggregation and Multiple Functional Annotation Methods.Frontiers in genetics · 2020Article
- Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis.Cell journal · 2019Article
- Iran's Contribution to Human Proteomic Research.Cell journal · 2019Review
- Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature.Stem cell research & therapy · 2019Article
- Transition of inner cell mass to embryonic stem cells: mechanisms, facts, and hypotheses.Cellular and molecular life sciences : CMLS · 2019Review
- Metabolic Signature of Pluripotent Stem Cells.Cell journal · 2018Article
- The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.Stem cell reports · 2018Article
- Shaping Cell Fate: Influence of Topographical Substratum Properties on Embryonic Stem Cells.Tissue engineering. Part B, Reviews · 2018Review
- RNA surveillance by the nuclear RNA exosome: mechanisms and significance.Non-coding RNA · 2018Article
- Small RNA Sequencing Reveals Dlk1-Dio3 Locus-Embedded MicroRNAs as Major Drivers of Ground-State Pluripotency.Stem cell reports · 2017Article
- Specification of murine ground state pluripotent stem cells to regional neuronal populations.Scientific reports · 2017Article
- Nanog Dynamics in Mouse Embryonic Stem Cells: Results from Systems Biology Approaches.Stem cells international · 2017Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differentiation potential of pluripotent embryonic stem cells (ESCs) can be manipulated via serum and medium conditions for direct cellular development or to maintain a naïve ground state. The self-renewal state of ESCs can thus be induced by adding inhibitors of mitogen activated protein kinase (MAPK) and glycogen synthase kinase-3 (Gsk3), known as 2 inhibitors (2i) treatment. We have used a shotgun proteomics approach to investigate differences in protein expressions between 2i- and serum-grown mESCs. The results indicated that 164 proteins were significantly upregulated and 107 proteins downregulated in 2i-grown cells compared to serum. Protein pathways in 2i-grown cells with the highest enrichment were associated with glycolysis and gluconeogenesis. Protein pathways related to organ development were downregulated in 2i-grown cells. In serum-grown ESCs, protein pathways involved in integrin and focal adhesion, and signaling proteins involved in the actin cytoskeleton regulation were enriched. We observed a number of nuclear proteins which were mostly involved in self-renewal maintenance and were expressed at higher levels in 2i compared to serum - Dnmt1, Map2k1, Parp1, Xpo4, Eif3g, Smarca4/Brg1 and Smarcc1/Baf155. Collectively, the results provided an insight into the key protein pathways used by ESCs in the ground state or metastable conditions through 2i or serum culture medium, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.