ArticleScientific reports2015
Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 185 citations in OpenAlex.
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- Immature olfactory sensory neurons are intrinsically excitable and show maturation-dependent changes in voltage-gated NaPflugers Archiv : European journal of physiology · 2026Article
- S3RL: Enhancing Spatial Single-Cell Transcriptomics With Separable Representation Learning.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A neuron type-specific microexon in Ank3/ankyrin-G modulates calcium activity and neuronal excitability.Nature communications · 2026Article
- A neuron type-specific microexon inbioRxiv : the preprint server for biology · 2025Article
- Glomeruli of the Mouse Olfactory Bulb: Numbers, Sizes, Shapes.The European journal of neuroscience · 2025Article
- Rapid Dissection and Dissociation of the Mouse Olfactory Epithelium for Single-Nucleus Suspensions.Journal of visualized experiments : JoVE · 2025Article
- A common mechanism of singular gene choice is revealed by broadly expressed odorant receptor transgenes within the olfactory epithelium.Cell reports · 2025Article
- Analysis of changes in intercellular communications in Alzheimer's disease reveals conserved changes in glutamatergic transmission in mice and humans.Scientific reports · 2025Article
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- scRNA-seq reveals transcriptional plasticity of var gene expression in Plasmodium falciparum for host immune avoidance.Nature microbiology · 2025Article
- Genomic snowflakes: how the uniqueness of DNA folding allows us to smell the chemical universe.Current opinion in genetics & development · 2025Review
- Cystic fibrosis alters the structure of the olfactory epithelium and the expression of olfactory receptors affecting odor perception.Science advances · 2025Article
- Visualizing the human olfactory projection and ancillary structures in a 3D reconstruction.Communications biology · 2024Article
- Establishment and maintenance of random monoallelic expression.Development (Cambridge, England) · 2024Review
- Identifying candidate genes underlying isolated congenital anosmia.Clinical genetics · 2024Article
- Epigenetic programming of stochastic olfactory receptor choice.Genesis (New York, N.Y. : 2000) · 2024Review
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45 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mouse olfactory mucosa is a complex chemosensory tissue composed of multiple cell types, neuronal and non-neuronal. We have here applied RNA-seq hierarchically, in three steps of decreasing cellular heterogeneity: starting with crude tissue samples dissected from the nose, proceeding to flow-cytometrically sorted pools of mature olfactory sensory neurons (OSNs), and finally arriving at single mature OSNs. We show that 98.9% of intact olfactory receptor (OR) genes are expressed in mature OSNs. We uncover a hitherto unknown bipartition among mature OSNs. We find that 19 of 21 single mature OSNs each express a single intact OR gene abundantly, consistent with the one neuron-one receptor rule. For the 9 single OSNs where the two alleles of the abundantly expressed OR gene exhibit single-nucleotide polymorphisms, we demonstrate that monoallelic expression of the abundantly expressed OR gene is extremely tight. The remaining two single mature OSNs lack OR gene expression but express Trpc2 and Gucy1b2. We establish these two cells as a neuronal cell type that is fundamentally distinct from canonical, OR-expressing OSNs and that is defined by the differential, higher expression of 55 genes. We propose this tiered experimental approach as a paradigm to unravel gene expression in other cellularly heterogeneous systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.