Evidence map›Paper›PMID 26670691›Full record

ArticleNeurogastroenterology and motility2016

Genome-wide DNA methylation profiling of peripheral blood mononuclear cells in irritable bowel syndrome.

S Mahurkar, C Polytarchou, D Iliopoulos, C Pothoulakis, E A Mayer, L Chang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurogastroenterology and motility, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 41 citations in OpenAlex.

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  11. Epigenetic modulation of visceral nociception.Neurogastroenterology and motility · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

S MahurkarOppenheimer Center for Neurobiology of Stress at UCLA, Division of Digestive Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
C PolytarchouCenter for Systems Biomedicine, Division of Digestive Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
D IliopoulosCenter for Systems Biomedicine, Division of Digestive Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
C PothoulakisDepartment of Medicine, Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
E A MayerOppenheimer Center for Neurobiology of Stress at UCLA, Division of Digestive Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
L ChangOppenheimer Center for Neurobiology of Stress at UCLA, Division of Digestive Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
University of California, Los Angeles · USCenter for Systems Biology · US

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
Women's Health and Functional Visceral Disorders CenterP50DK064539 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MAYER, EMERAN A · 2002 to 2017
$17.5M
UCLA CLINICAL AND TRANSLATIONAL SCIENCE INSTITUTEUL1RR033176 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2011 to 2011
$15.5M
PERCEPTION AND MODULATION OF VISCERAL SENSATIONSR01DK048351 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MAYER, EMERAN A · 1996 to 2019
$8.2M
DNA Methylation Based Biomarkers and Epigenetic Regulation in IBSR21DK104078 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHANG, LIN · 2015 to 2016
$424k
NCRR NIH HHS 1 UL1 RR033176NCRR NIH HHS UL1 RR033176NIDDK NIH HHS P30 DK041301NIDDK NIH HHS P30 DK 41301NIDDK NIH HHS P50 DK064539NIDDK NIH HHS R01 DK048351NIDDK NIH HHS R21 DK104078NIDDK NIH HHS R21 DK104078-01A1
6 · The paper itself

Abstract

backgroundIrritable bowel syndrome (IBS) is a stress-sensitive disorder. Environmental factors including stress can trigger epigenetic changes, which have not been well-studied in IBS. We performed a pilot study investigating genome-wide DNA methylation of IBS patients and healthy controls (HCs) to identify potential epigenetic markers and associated pathways. Additionally, we investigated relationships of epigenetic changes in selected genes with clinical traits.

methodsTwenty-seven IBS patients (59% women; 10 IBS-diarrhea, 8 IBS-constipation, 9 IBS-mixed) and 23 age- and sex-matched HCs were examined. DNA methylation from peripheral blood mononuclear cells (PBMCs) was measured using HM450 BeadChip, and representative methylation differences were confirmed by bisulphite sequencing. Gene expression was measured using quantitative PCR. Gastrointestinal (GI) and non-GI symptoms were measured using validated questionnaires. Associations were tested using non-parametric methods. KEY

resultsGenome-wide DNA methylation profiling of IBS patients compared with HCs identified 133 differentially methylated positions (DMPs) (mean difference ≥10%; p < 0.05). These genes were associated with gene ontology terms including glutathione metabolism related to oxidative stress and neuropeptide hormone activity. Validation by sequencing confirmed differential methylation of subcommissural organ (SCO)-Spondin (SSPO), glutathione-S-transferases mu 5 (GSTM5), and tubulin polymerization promoting protein genes. Methylation of two promoter CpGs in GSTM5 was associated with epigenetic silencing. Epigenetic changes in SSPO gene were positively correlated with hospital anxiety and depression scores in IBS patients (r > 0.4 and false discovery rate <0.05). CONCLUSIONS & INFERENCES: This study is the first to comprehensively explore the methylome of IBS patients. We identified DMPs in novel candidate genes which could provide new insights into disease mechanisms; however, these preliminary findings warrant confirmation in larger, independent studies.

Indexed as

DNA MethylationAdultFemaleGenome-Wide Association StudyHumansIrritable Bowel SyndromeLeukocytes, MononuclearMaleOligonucleotide Array Sequence AnalysisPilot ProjectsPolymerase Chain ReactionStress, Physiologicalbisulphite sequencingDNA methylationhuman methylation 450 arrayirritable bowel syndromeoxidative stress

Identifiers

PMID26670691
PMCPMC4760882
OpenAlexW2207816086

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.